指導教授：張祖亮臺灣大學：園藝學研究所侯宗翰Hou, Tsung-HanTsung-HanHou2014-11-292018-06-292014-11-292018-06-292013http://ntur.lib.ntu.edu.tw//handle/246246/263089最終醣化蛋白(advanced glycation end-products, AGEs)的形成，導致發炎反應的發生與胰島素抗性(insulin resistance)的產生。有證據顯示抗氧化劑可以抑制活性氧(reactive oxygen species, ROS)的形成，經由抑制糖基化(glycation)減少醣化終產物的生成。龍葵(Solanum nigrum)可以作為改善血糖的藥用食物，然而其活性成分與作用機轉仍然不清楚。本研究結果顯示，龍葵95%乙醇萃取物的抗氧化活性高於龍葵50%乙醇萃取物和龍葵水萃取物。此外，龍葵95%乙醇萃取物具有抑制糖基化的活性，可以抑制果糖胺(fructosamine)與α-二羰基化合物(α-dicarbonylcompiund)的生成。在每毫克龍葵95%乙醇萃取物中，solasonine與solamargine的量分別為0.484毫克和0.183毫克。基於上述，龍葵具備當作萃取抗糖基化和抗糖尿病活性成分的潛力。Solanum nigrum are recently not only considered to be the brand-new source of functional ingredients that exerts antiglycation and anti-diabetes activities but also to be kind of medicinal food for improving blood glucose. However, there are only few identities of the active compounds and how those above-mentioned compounds counteract diabetes are still unknown. To induces insulin resistance occurs in an inflammatory response that requires the hyperglycemia results in the formation of advanced glycation end-products (AGEs). Evidence indicates that antioxidants can suppress the formation of reactive oxygen species, decrease levels of AGEs by inhibiting glycation. In this research demonstrates that 95% ethanolic extracts of Solanum nigrum exerted significant antioxidative activity compared with 50% ethanolic extracts and aqueous extracts. Moreover, 95% ethanolic extracts of S. nigrum produced antiglycative activity, which contributed to the inhibition of fructosamine and generation of α-dicarbonyl compounds. The results indicate that 95% ethanolic extracts of S. nigrum are with great bioactivities on antiglycation and anti-diabetes.口試委員會審定書....................................................# 謝誌................................................................i 中文摘要...........................................................ii 英文摘要..........................................................iii 目錄...............................................................iv 第一章 前言.........................................................1 第二章 文獻回顧.....................................................4 2.1 龍葵簡介........................................................4 2.2 抗氧化作用......................................................6 2.3 抗氧化物........................................................7 2.4 生物體對於抗氧化作用有其依賴...................................12 2.5 抗氧化酶系統...................................................14 2.6 食品中的抗氧化劑...............................................15 2.7 醣化作用.......................................................16 第三章 材料與方法..................................................20 3.1 藥品...........................................................20 3.2 樣品製備.......................................................21 3.3 DPPH自由基清除活性測定........................................21 3.4 總抗氧化活性測定...............................................21 3.5 還原力活性測定.................................................22 3.6 超氧陰離子自由基清除能力測定...................................22 3.7 一氧化氮自由基清除活性測定.....................................22 3.8 抗糖基化活性測定...............................................23 3.9 果糖胺生成測定(Amadori產物) ...................................23 3.10α-二羰基化合物生成測定........................................24 3.11高效能液相層析儀分析..........................................24 3.12統計分析......................................................25 第四章 結果與討論..........................................................26 第五章 參考文獻.................................................................40 附錄 已發表之SCI期刊…………..............…………………….47 圖目錄 圖1. 不同龍葵萃取物DPPH自由基清除能力測定..................31 圖2. 不同龍葵萃取物總抗氧化能力測定........................32 圖3. 不同龍葵萃取物還原能力測定............................33 圖4. 不同龍葵萃取物一氧化氮清除能力測定....................34 圖5. 不同龍葵萃取物超氧陰離子清除能力測定..................35 圖6. 高效能液相層析儀分析..................................36 圖7. 龍葵95%乙醇萃取物、solasonine與solamargine抑制醣化終產物生成能力測定.....................................................37 圖8. 龍葵95%乙醇萃取物、solasonine與solamargine抑制果糖胺生成能力測定.........................................................38 圖9. 龍葵95%乙醇萃取物、solasonine與solamargine抑制α-二羰基化合物生成能力測定...............................................391680721 bytesapplication/pdf論文公開時間：2014/01/27論文使用權限：同意有償授權(權利金給回饋學校)最終醣化蛋白抗氧化活性龍葵抗醣化作用抗糖尿病[SDGs]SDG3thesishttp://ntur.lib.ntu.edu.tw/bitstream/246246/263089/1/ntu-102-R99628143-1.pdf