2012-02-212024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/660088摘要：植物生育期間經常受到病原真菌、細菌等之危害，故常藉由施用化學藥劑進行病害防治；但病原菌容易產生抗藥性，需以其他替代方案協助病害問題之解決。使用天然抗微生物資材替代化學藥劑進行保護種子、種苗與植株，即為一降低病害發生之策略。本實驗室在百合水楊酸誘導表現訊息核酸之 cDNA 選殖工作中，鑑定一與植物防禦有關之解碼富含甘胺酸與半胱氨酸蛋白質的基因 - LsGRP1，經測試已知 LsGRP1 蛋白片段具有廣效之抗微生物活性及抑制病害的作用。本年度擬針對十字花科黑斑病及其真菌病原，分析 LsGRP1 C 端胜&#32957;對不同種病原菌及菌株孢子發芽之抑制作用，以了解 LsGRP1 C 端胜&#32957;在不同寄主上應用以抑制黑斑病菌的可行性；並測試不同處理時間下，LsGRP1 C 端胜&#32957;用以防治十字花科作物黑斑病的效果，由網室田間試驗觀察實際應用的可行性及了解合理施用 LsGRP1 C 端胜&#32957;的方式。 <br> Abstract: Chemical control is regularly applied in plant protection to decrease bacterial and fungal diseases. However, there are many resistant pathogens evolved under the stress of chemical treatment. To solve the problem, there is a strategy to reduce chemicals by using natural antimicrobial materials for seed and seedling protection. LsGRP1 is a salicylic acid-responsive gene encoding a glycine- and cystein-rich protein present in plant extracellular matrix. LsGRP1 C-terminal peptide had been shown to be an antimicrobial peptide and can be a protectant against disease development. In this year, the antifungal activity of LsGRP1 C-terminal peptide will be examined on different species and isolates of Alternaria pathogens of cruciferous crops by germination assay. The effect of LsGRP1 C-terminal peptide to protect cruciferous plants against black spot diseases will be examined in the screenhouse and field assay to evaluate the applicability of LsGRP1 C-terminal peptide in crucifer production and the suitable way of application.十字花科作物黑斑病葵百合富含甘胺酸蛋白CrucifersBlack spot diseaseLsGRP1