Huang M.-L.Chiang P.-S.Chia M.-Y.Luo S.-T.LUAN-YIN CHANGLin T.-Y.Ho M.-S.Lee M.-S.2020-12-152020-12-1520131935-2727https://www.scopus.com/inward/record.uri?eid=2-s2.0-84874778618&doi=10.1371%2fjournal.pntd.0002067&partnerID=40&md5=54d58380878d36742e7f0550e5941900https://scholars.lib.ntu.edu.tw/handle/123456789/525559Background: Recently, enterovirus 71 (EV71) has caused life-threatening outbreaks involving neurological and cardiopulmonary complications in Asian children with unknown mechanism. EV71 has one single serotype but can be phylogenetically classified into 3 main genogroups (A, B and C) and 11 genotypes (A, B1~B5 and C1~C5). In Taiwan, nationwide EV71 epidemics with different predominant genotypes occurred in 1998 (C2), 2000-2001 (B4), 2004-2005 (C4), and 2008 (B5). In this study, sera were collected to measure cross-reactive neutralizing antibody titers against different genotypes. Methods: We collected historical sera from children who developed an EV71 infection in 1998, 2000, 2005, 2008, or 2010 and measured cross-reactive neutralizing antibody titers against all 11 EV71 genotypes. In addition, we aligned and compared the amino acid sequences of P1 proteins of the tested viruses. Results: Serology data showed that children infected with genogroups B and C consistently have lower neutralizing antibody titers against genogroup A (>4-fold difference). The sequence comparisons revealed that five amino acid signatures (N143D in VP2; K18R, H116Y, D167E, and S275A in VP1) are specific for genogroup A and may be related to the observed antigenic variations. Conclusions: This study documented antigenic variations among different EV71 genogroups and identified potential immunodominant amino acid positions. Enterovirus surveillance and vaccine development should monitor these positions. ? 2013 Huang et al.[SDGs]SDG3neutralizing antibody; vaccine; amino acid sequence; antibody response; antigenic variation; article; child; clinical article; cross reaction; Enterovirus 71; Enterovirus infection; genotype; human; infant; neurological complication; newborn; nucleotide sequence; polymerase chain reaction; preschool child; school child; sequence analysis; serodiagnosisjournal article10.1371/journal.pntd.00020672-s2.0-84874778618