2018-06-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/652588摘要：作物病害為亟需解決之重大問題，而危害全球眾多經濟作物且極難防治之青枯病、細菌性軟腐病及灰黴病更是重要，且與非生物逆境反應有密切交互作用。本研究團隊在植物-青枯病菌(Ralstonia solanacearum, Rs) 交互作用已有多年研究經驗，現已確認Rs之特定效應蛋白(effectors)進入植物細胞後會增加或抑制茄科植物對數種病菌之抗性。本計畫擬利用這些effectors作為工具，探討解植物天然防禦機制，並與其他研究計畫整合，以利研擬對環境友善之作物保護措施。我們將分析特定effectors對番茄/煙草防禦重要病害與非生物性逆境之影響、闡述其防禦訊息傳調路徑、找尋與其具直接交互作用與被調控之目標基因/蛋白質以瞭解其具體調控機制、闡明關鍵調控基因之功能與應用潛力。藉由各計畫間之密切整合，本研究預期將獲得植物防禦機制之重要資訊，並匯集可能有助於作物農業生技發展之資源。<br> Abstract: Crop diseases are urgent problems to be solved worldwide, particualrly those affecting wide-range of economically important corps including bacterial wilt (caused by Ralstonia solanacearum or Rs), bacterial soft rot and gray leaf mold. Extensive cross-talks also exist between plant resposnes to these pathogens and abiotic stresses. Our team has been studying plant-Rs interactions for many years and our current results show that several Rs effector proteins can promote or reduce plant defense to a few pathogens. Through the intensive coordiation with other subprojects, this study aims to carry out the following work in order to elucidate plant natural defense mechanisms and establish green disease-control means. We will determine the spectrum of effectors-modulated Solanaceae responses to various important pathogens and abiotic stresses, decipher the involved defense signaling pathways, decipher the underlying regulatory mechanisms by identifying the interactors and regulated genes/targets of the effectors, and determine the functions and application potentials of these genes. Through the integration of this Programmatic Project, the proposed studies are projected to gather important information on plant defense mechanisms and collect resources valuable for crop biotechnology.植物抗病植物逆境細菌效應蛋白plant disease defenseplant stressbacterial effector