Lin C.-CSHIH-SHUN LINChen T.-C.2022-12-142022-12-14202203048608https://www.scopus.com/inward/record.uri?eid=2-s2.0-85129390026&doi=10.1007%2fs00705-022-05449-z&partnerID=40&md5=4ad0214692d3621779d38be949f6ec42https://scholars.lib.ntu.edu.tw/handle/123456789/626027In April 2011, a virus was isolated by single-lesion isolation on Chenopodium quinoa leaves from an amaryllis plant with chlorotic ringspots in a private garden in Changhua County, Taiwan. An Illumina MiSeq sequencing system was used to determine the genomic nucleotide (nt) sequence of the virus. A de novo-assembled contig with 9377 nt, containing an open reading frame encoding a putative potyviral polyprotein, was annotated as the potyvirus Amazon lily mosaic virus (ALiMV), sharing 95.5% nt sequence identity with a partial genomic sequence of ALiMV available in the GenBank database. Therefore, the amaryllis virus was designated as ALiMV-TW. Through 5´ and 3´ rapid amplification of cDNA ends (RACE), the complete 9618-nt genome sequence of ALiMV-TW was determined. Sequence comparisons indicated that the genome and polyprotein of ALiMV-TW share 52.3–65.1% nt and 30.1–64.2% aa sequence identity, respectively, with those of other potyviruses. This is the first report of a complete genome sequence of ALiMV. © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.polyprotein; Amaryllidaceae; genetics; lily; phylogeny; Potyvirus; virus genome; Amaryllidaceae; Genome, Viral; Lilium; Phylogeny; Polyproteins; Potyvirusjournal article10.1007/s00705-022-05449-z354820882-s2.0-85129390026