Yang, Shu-JyuanShu-JyuanYangPai, Jui-AnJui-AnPaiYao, Cheng-JungCheng-JungYaoHuang, Chung-HuanChung-HuanHuangJENNY LING-YU CHENChing-Hua WangKE-CHENG CHENMING-JIUM SHIEH2023-03-172023-03-1720231868-6958https://scholars.lib.ntu.edu.tw/handle/123456789/629364Background: Chemo-radiotherapy is the combined chemotherapy and radiotherapy on tumor treatment to obtain the local radiosensitization and local cytotoxicity of the tumor and to control the microscopic metastatic disease. Methods: In this study, 7-ethyl-10-hydroxycamptothecin (SN38) molecules could be successfully loaded into human serum albumin (HSA)–hyaluronic acid (HA) nanoparticles (SH/HA NPs) by the hydrophobic side groups of amino acid in HSA. Results: HSA could be used to increase the biocompatibility and residence time of the nanoparticles in the blood, whereas HA could improve the benefits and overall treatment effect on CD44-expressing colorectal cancer (CRC), and reduce drug side effects. In addition to its role as a chemotherapeutic agent, SN38 could be used as a radiosensitizer, able to arrest the cell cycle, and allowing cells to stay in the G2/M stage, to improve the sensitivity of tumor cells to radiation. In vivo results demonstrated that SH/HA NPs could accumulate in the tumor and produce significant tumor suppression, with no adverse effects observed when combined with γ-ray irradiation. This SH/HA NPs-medicated chemo-radiotherapy could induce an anti-tumor immune response to inhibit the growth of distal tumors, and produce an abscopal effect. Conclusions: Therefore, this SN38-loaded and HA-incorporated nanoparticle combined with radiotherapy may be a promising therapeutic artifice for CRC in the future.enChemo-radiotherapy; 7-Ethyl-10-hydroxycamptothecin; Human serum albumin; Hyaluronic acid; Abscopal effect; DRUG-DELIVERY; ENHANCED PERMEABILITY; ALBUMIN NANOPARTICLES; OXIDE NANOPARTICLES; CELLS; SENSITIVITY; IRINOTECAN; PRODRUG; KINASE; CPT-11[SDGs]SDG3journal article10.1186/s12645-022-00151-w2-s2.0-85145461927WOS:000906713100001https://api.elsevier.com/content/abstract/scopus_id/85145461927