Ravindran, AnitaAnitaRavindranAdav, SunilSunilAdavYang, Shang-ShyngShang-ShyngYang2012-10-192018-07-062012-10-192018-07-062010http://ntur.lib.ntu.edu.tw//handle/246246/243156A functional hydrogen producing consortium was isolated from soil by heat pre-treatment technique and hydrogen production at different substrate concentration was evaluated. The forest soil was heat pre-treated at 65, 80, 95, 105 and 120 °C temperature for 1 h. As revealed by PCR-DGGE analysis and hydrogen yield, the hydrogen producing microbial community changed with increase in heat pre-treatment temperatures giving potential hydrogen producing consortium at 95-105 °C soil pre-treatment. The maximum hydrogen production rate, hydrogen yield and cumulative hydrogen with 15-20 g glucose were 1390-1576 mL/L/day, 1.83-1.93 mol H2/mol glucose, and 2966-3146 mL H2/L, respectively. The metabolic pathways shifted from ethanol-type to acetate-formate type as soil pre-treatment temperature increased from 65 to 120 °C. The soil heat pre-treatment approach is effective for isolating hydrogen producing natural Clostridium consortium from the soil as enumerations of the functional strains need specific temperature range to florish. © 2010 Elsevier Ltd.en-USForest soil; Functional consortium; Heat pre-treatment; Hydrogen production[SDGs]SDG7[SDGs]SDG15AS-soils; Different substrates; Forest soils; Functional consortium; Functional strain; Hydrogen production rate; Hydrogen yields; Metabolic pathways; Microbial communities; PCR-DGGE; Pre-Treatment; Pretreatment temperature; Temperature range; Ethanol; Gas producers; Glucose; Soils; Hydrogen production; bacterium; forest soil; heating; hydrogen; metabolism; microbial community; polymerase chain reaction; soil microorganism; temperature effect; Clostridiumjournal article10.1016/j.renene.2010.04.010http://ntur.lib.ntu.edu.tw/bitstream/246246/243156/-1/110.pdf