2023-01-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/653898高麗菜 (Brassica oleracea var. capitate) 為十字花科植物甘藍菜變種之一，又名包心菜、捲心菜。高麗菜營養成分多樣，富含膳食纖、微量元素、維生素，以及多種硫代配醣體 (Glucosinolates, GSs)。 其中吲哚類 GSs 已被文獻證實具有良好抗氧化能力，可以預防動脈硬化、脂肪肝及抗腫瘤等益生功效。新鮮高麗菜水分含量高，在儲藏過程中容易因微生物或自體酵素作用而產生各種生理上之化學變化，大幅影響產品的保鮮儲藏期限。本計畫擬研究國產大宗蔬果及特用作物之加工技術改良與研發，並結合發酵工程篩選適合高麗菜發酵之乳酸菌，透過此乳酸菌發酵達成保留原十字花科植物中的 GSs 化合物。除此之外也將透過本團隊之固態發酵技術放大生物反應器生產規模，縮短發酵所需時間，同時建立檢測 GSs 技術平台，並以體外細胞模式評估其對於抑制體脂肪形成之益生功效。在完成益生乳酸高麗菜原型產品後，後續將進行以酸高麗菜乾為基底之休閒食品開發，預期可推廣至更多消費者群體。 Brassica oleracea var. capitate is one of the cruciferous vegetable varieties, also known as cabbage and cabbage. Cabbage has many nutritional components, and is rich in dietary fiber, trace elements, vitamins, and various kinds of glucosinolates (GSs). Among them, indole GSs have been proved to have good antioxidant activity, which can prevent arteriosclerosis, fatty liver, and anti-tumor probiotic effects. Fresh cabbage has high water content, and it is easy to produce significant physiological and chemical changes due to the action of microorganisms or enzymatic degradation during storage. Those reactions greatly affect the shelf life of the product. This project intends to study the development of processing technology and the selection of suitable lactic acid bacteria for cabbage fermentation for specific vegetables. By combining vegetable pickling processing with fermentation engineering, the total glucosinolates retention rate of sour cabbage fermented by lactic acid bacteria could be increased. In addition, the production scale of the bioreactor will be enlarged through the solid-state fermentation technology of our research team. At the same time, a detection platform for GSs was established, and its effect on in vitro inhibiting body fat accumulation was evaluated via a cell model. After the production of the prototype product is completed, the development of snack food based on dried sour cabbage is expected to be extended to more consumer groups and create business value.高麗菜;硫代配糖體;不易形成體脂肪;生物反應器;Cabbage ;glucosinolates; antiobesity; bioreactor