2012-04-142024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/681466摘要：本研究利用法國研究船航次 MD 178-10 於台灣西南海域所鑽取之超長岩芯進行微生物族群分析。過去地球物理與化學的探勘資料顯示，此海域可能蘊含豐富的天然氣水合物，並形成一獨特的硫酸鹽-甲烷介面 (sulfate-methane interface, SMI)，此地球化學介面的形成是由於甲烷氧化古菌與硫酸鹽還原菌形成共生關係，利用向上遷移的甲烷與海水的硫酸鹽，獲取代謝所需能量所致。為了瞭解於這一介面上詳細的微生物族群結構，本研究選取分布於被動大陸邊緣、上部斜坡及下部斜坡共 18 根岩芯中的 SMI 區段，進行 16S rRNA 基因的多樣性與數量分析。本研究將利用焦磷酸定序法 (pyrosequencing) 建立微生物基因庫，並利用即時定量聚合&#37238;連鎖反應 (quantitative PCR，qPCR) 觀察微生物族群的豐度。我們預期能分析出微生物族群結構於不同地理位置 SMI 區域的差異。<br> Abstract: This study attempts to characterize microbial community structures associated with sediments retrieved by coring in offshore southwestern Taiwan during the MD 178-10 cruise. Previous geophysical and geochemical explorations indicate that the region potentially bear abundant gas hydrates and is characterized by a sulfate-methane interface (SMI). The SMI is commonly interpreted as the interface where syntrophic anaerobic methane oxidizing archaea and sulfate reducing bacteria metabolize the upward migrating methane and downward percolating seawater sulfate. To unravel the detailed microbial community structures at this interface, this study analyzes the diversity and abundance of 16S rRNA genes in sediments at the SMI from 18 cores distributing over passive margin, upper slope and lower slope. High throughput pyrosequencing will be used to construct the gene libraries, whereas quantitative PCR will be used to determine the gene abundances. We anticipate to reveal the variations in community structure in geographically different regions.微生物族群天然氣水合物microbial communitygas hydrate