Hovhannisyan, V.V.HovhannisyanGuo, H.W.H.W.GuoHovhannisyan, A.A.HovhannisyanGhukasyan, V.V.GhukasyanBuryakina, T.T.BuryakinaChen, Y.F.Y.F.ChenCHEN-YUAN DONGYANG-FANG CHEN2019-12-192019-12-192014https://scholars.lib.ntu.edu.tw/handle/123456789/436299Collagen is the main structural protein and the key determinant of mechanical and functional properties of tissues and organs. Proper balance between synthesis and degradation of collagen molecules is critical for maintaining normal physiological functions. In addition, collagen influences tumor development and drug delivery, which makes it a potential cancer therapy target. Using second harmonic generation, two-photon excited fluorescence microscopy, and spectrofluorimetry, we show that the natural pigment hypericin induces photosensitized destruction of collagen-based tissues. We demonstrate that hypericin-mediated processes in collagen fibers are irreversible and may be used for the treatment of cancer and collagen-related disorders. ?2014 Optical Society of America.[SDGs]SDG3Drug delivery; Fluorescence microscopy; Fluorescence spectroscopy; Histology; Nonlinear optics; Synthesis (chemical); Tissue; Collagen molecules; Functional properties; Multi-photon microscopy; Photoinduced process; Physiological functions; Spectrofluorimetry; Structural proteins; Two-photon excited fluorescence microscopies; Collagen; collagen fiber; gelatin; hypericin; angiogenesis; animal tissue; article; cattle; chicken; collagen disease; controlled study; fluorescence spectroscopy; illumination; multiphoton microscopy; nonhuman; photostimulation; rejuvenation; skin sensitization; spectrofluorometry; tendon; tumor growth; ultraviolet radiation; wound healingjournal article10.1364/BOE.5.0013552-s2.0-84899759670https://www.scopus.com/inward/record.uri?eid=2-s2.0-84899759670&doi=10.1364%2fBOE.5.001355&partnerID=40&md5=158bb0f69bc90478dfc59304358e55fc