KO-JEN LILu, Ming-ChiMing-ChiLuSONG-CHOU HSIEHCHENG-HAN WUYu, Hsin-SuHsin-SuYuTsai, Chang-YouhChang-YouhTsaiCHIA-LI YU2022-02-102022-02-102006-080741-5400https://scholars.lib.ntu.edu.tw/handle/123456789/594016It is conceivable that a membrane component(s) is transferred from antigen-presenting cells to T cells after antigenic stimulation. However, it is not clear whether a certain membrane component(s) is transferred from polymorphonuclear neturophils (PMN) to T cells for immunomodulation. In the presence study, we cocultured two of the three autologous cells-PMN, CD4+ T, and red blood cells (RBC)-homotypically or heterotypically for 1 h. Spontaneous membrane exchange between autologous PMN-PMN and PMN-CD4+ T but not between CD4+ T-CD4+ T or RBC-CD4+ T was observed with a confocal microscope. Loss of membrane exchange between two paraformaldehyde-fixed cells suggests that mutual membrane exchange is via cell-cell contact. Different combinations of cellular enzyme-linked immunosorbent assay for measuring the binding between fixed cells and biotinylated cell lysates showed the same tendency. To identify the molecule(s) mediating PMN-CD4+ T binding, we compared the banding of biotinylated PMN lysates and the banding of plain PMN lysate probed by biotinylated CD4+ T lysate in 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. We found that a 75- to 80-kDa surface-expressed molecule on PMN exists constantly to mediate PMN-CD4+ T binding. Peptide analysis disclosed that the molecule had 99.8% identity with lactoferrin (LF). The expression of LF on system lupus erythematosis (SLE)-PMN is less than normal PMN. PMN-CD4+ T coculture increased LF expression on CD4+ T. Normal PMN and human milk-derived LF suppressed interferon-gamma (IFN-gamma) but enhanced interleukin (IL)-10 production of anti-CD3+anti-CD28-activated, normal CD4+ T. In contrast, coculture of SLE-PMN and autologous CD4+ T suppressed IFN-gamma and IL-10 production. These results suggest that the surface-expressed LF released from PMN after contact with autologous CD4+ T modulated its T helper cell type 1 (Th1)/Th2 cytokine production. Decreased LF expression on SLE-PMN abnormally modulates Th1/Th2 production by CD4+ T cells.ensystemic lupus erythematosus; interferon-gamma; interleukin-10; DELAYED-TYPE HYPERSENSITIVITY; INVIVO IMMUNE-RESPONSE; RED-BLOOD-CELLS; MHC CLASS-II; SELECTIVE DEPLETION; MONOCLONAL-ANTIBODY; T-CELLS; RP-3 TREATMENT; INTERCELLULAR TRANSFER; RHEUMATOID-ARTHRITISjournal article10.1189/jlb.1105668167697652-s2.0-33748878225WOS:000243015600016https://scholars.lib.ntu.edu.tw/handle/123456789/542216