2008-01-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/652330摘要：台灣重要柑橘系統性病原包括黃龍病細菌(HLBB)、萎縮病毒(CTV)、破葉病毒(CTLV)與鱗砧次病毒(CEVd)等，這些病原經由帶病種苗普遍傳播而危害嚴重，生產無病種苗搭配敏感且正確的檢疫技術乃是防治這些病害的首要之道。本計畫以原本初步研發成功的柑橘病原檢疫技術為基礎，進一步再研發與改善，目標在於建立一套方便且快速的整合性偵測套組。具體工作項目包括：1.應用Real-time PCR技術研發黃龍病細菌(HLBB)、萎縮病毒(CTV)、破葉病毒(CTLV)與鱗砧次病毒(CEVd)的分子定量偵測法；2. HLBB、CTV、CTLV與CEVd整合性PCR/RT-PCR快速偵測套組之研發；3.柑橘萎縮病毒與破葉病毒的世界通用型快速抗體偵測試劑之研製；4. 柑橘黃龍病原之單株抗體及診斷試劑研製；5.柑橘萎縮病毒系統特異性偵測試劑的研發等。所得研究成果不但可供國內防檢疫之應用，更因這些病原皆為國際性重要病原，未來可申請專利而推展於國際。<br> Abstract: The systemic pathogens such as the bacteria causing citrus huanglongbing, Citrus tristeza virus (CTV), Citrus tatter leaf virus (CTLV) and Citrus exocortis viroid (CEVd) are predominant and destructive to citrus industry in Taiwan. These pathogens are mainly transmitted by vegetative propagation. Establishment of pathogen-free nursery systems and development of rapid and sensitive diagnostic probes are considered to be the most important methods for the pest control. This research project is dedicated to develop new diagnostic probes and improve several devised diagnostic methods using immunological and molecular techniques. An integrated diagnostic kit will be produced through combination of all our devised detection methods for these citrus systemic pathogens. Several items will be conducted this year: (1) application of the Real-time PCR technique in the development of molecular quantitative detections for huanglongbing bacteria (HLBB), CTV, CTLV and CEVd；(2) combination of PCR/RT-PCR in one step for the simultaneous detection of HLBB, CTV, CTLV and CEVd; (3) development of antibodies against the common epitops of CTV and CTLV for production of diagnostic probes; (4) preparation of the monoclonal antibodies against HLBB; (5) development of the diagnostic probes for identification of different strains of CTV. These devised methods will be helpful to the inspection and management of the citrus pests, and it is worthy of international patent for world-wide extension.柑橘黃龍病細菌柑橘萎縮病毒柑橘破葉病毒柑橘鱗砧次病毒citrus huanglongbingCitrus tristeza virus (CTV)Citrus tatter leaf virus (CTLV)Citrus exocortis viroid (CEVd)