2007-01-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/701823摘要：本計畫基於Bacillus cereus可以經由處理根圈誘導植物系統性抗病的能力，希望開發相關之附屬價值。擬以B. cereus菌株處理百合及接種灰黴病菌，篩選具有差異性表現特性之cDNA選殖株；並進行北方雜合分析，確認相關基因會受B. cereus處理及灰黴病菌感染之刺激而有差異之表現；選擇目標基因進行全長cDNA選殖及嘗試建構轉基因植物，瞭解其應用於建構轉基因抗病品系基因資源之潛力。另一方面，嘗試鑑定B. cereus菌株誘導植物抗病之因子，由轉位子誘變及番茄接種篩選試驗，篩選B. cereus之植物抗病誘導因子變異菌株，續由轉位子插入位置分析，鑑定與植物抗病誘導能力有關之基因，後續擬發展植物保護劑用途。<br> Abstract: This project is based on the ability of Bacillus cereus to induce disease resistance after application to the rhizosphere of plants and is aimed to develop the related merits. The cDNAs corresponding to differentially expressed genes will be isolated from lily leaves after B. cereus treatment and Botrytis elliptica inoculation. The trait of differential expression will be verified by Northern hybridization. The full-length target cDNAs will be cloned and used to construct transgenic plants to test their potential as the resources of resistance genes. On the other hand, the genes encoding elicitors of B. cereus will be identified by transposon mutagenesis. The transposon-tagged mutants will be screened for their inability to induce systemic resistance of plants by inoculation assay on tomato seedlings. The sites of transposon insertion will be determined by cloning and sequencing of the flanking sequence. The genes encoding for the putative elicitors will be cloned and developed further as plant protection agents.Bacillus cereus差異性表現cDNA選殖植物抗病基因資源轉位子誘變誘發因子Bacillus cereusdifferential expressioncDNA cloningplant disease resistancegene resourcestransposon mutagenesiselicitors