CHANG-FENG DAI2018-09-102018-09-102010http://www.scopus.com/inward/record.url?eid=2-s2.0-78650189387&partnerID=MN8TOARShttp://scholars.lib.ntu.edu.tw/handle/123456789/356865Eight new eunicellin-base diterpenoids, hirsutalins A-H (1-8), were isolated from the soft coral Cladiella hirsuta. Their structures were elucidated by spectroscopic methods, particularly in 1D and 2D NMR experiments. The absolute configuration of 1 was determined by Mosher's method. Compounds 1, 5, and 6 have been shown to exhibit cytotoxicity toward several cancer cell lines. Compounds 2-4 and 8 were found to display significant in vitro anti-inflammatory activity in LPS-stimulated RAW264.7 macrophage cells by inhibiting the expression of the iNOS protein, with compound 2 also effectively reducing the level of COX-2 protein. ? 2010 The American Chemical Society and American Society of Pharmacognosy.[SDGs]SDG3[SDGs]SDG14antiinflammatory agent; cyclooxygenase 2; cytotoxic agent; diterpenoid; hirsutalin a; hirsutalin b; hirsutalin c; hirsutalin d; hirsutalin e; hirsutalin f; hirsutalin g; hirsutalin h; unclassified drug; animal cell; antiinflammatory activity; article; cancer cell culture; carbon nuclear magnetic resonance; Cladiella hirsuta; coral; cytotoxicity; cytotoxicity test; drug isolation; drug structure; drug synthesis; heteronuclear multiple bond correlation; human; human cell; mouse; nonhuman; nuclear Overhauser effect; proton nuclear magnetic resonance; Animals; Anthozoa; Anti-Inflammatory Agents, Non-Steroidal; Antineoplastic Agents; Cyclooxygenase 2 Inhibitors; Diterpenes; Drug Screening Assays, Antitumor; Humans; Lipopolysaccharides; Macrophages; Mice; Molecular Structure; Nitric Oxide Synthase Type II; Nuclear Magnetic Resonance, Biomolecular; Alcyonacea; Cladiellajournal article10.1021/np100401f2-s2.0-78650189387WOS:000284559100008