2017-07-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/682022摘要：In eukaryotic cells, gene activity is controlled not only by DNA sequences but also by epigenetic marks that can be transmitted to a cell progeny during mitosis or meiosis. Epigenetic changes involve modifications of DNA activity by methylation, histone modifications or chromatin remodelling without alteration of the nucleotide sequence. Among the multiple epigenetic mechanisms that modify chromatin to regulate gene expression, histone modifications including acetylation, methylation, phosphorylation and ubiquitination play a major role. Our studies indicate that histone methylation and acetylation play an important role in plant response to environmental stress. In the proposed research, the molecular mechanisms of how histone modifications are involved in plant response to environmental stresses will be investigated. We will identify histone modification proteins involved in plant stress responses. The interaction of histone modification proteins with transcription factors and other chromatin remodeling proteins will be analyzed. Furthermore, the interacting proteins and protein complexes of histone modification proteins will be identified. Understanding how histone modifications regulate gene expression will contribute to our understanding of molecular mechanisms underlying the roles played by epigenetic regulation in plants. These studies will provide insights into the gene silencing, DNA methylation and other histone modification-associated gene regulation in plants. Given the importance of epigenetic mechanisms in the regulation of gene activities, a better understanding of how histone modifications modulate plant gene expression in plant stress responses may have wide application to crop improvement.<br> Abstract: In eukaryotic cells, gene activity is controlled not only by DNA sequences but also by epigenetic marks that can be transmitted to a cell progeny during mitosis or meiosis. Epigenetic changes involve modifications of DNA activity by methylation, histone modifications or chromatin remodelling without alteration of the nucleotide sequence. Among the multiple epigenetic mechanisms that modify chromatin to regulate gene expression, histone modifications including acetylation, methylation, phosphorylation and ubiquitination play a major role. Our studies indicate that histone methylation and acetylation play an important role in plant response to environmental stress. In the proposed research, the molecular mechanisms of how histone modifications are involved in plant response to environmental stresses will be investigated. We will identify histone modification proteins involved in plant stress responses. The interaction of histone modification proteins with transcription factors and other chromatin remodeling proteins will be analyzed. Furthermore, the interacting proteins and protein complexes of histone modification proteins will be identified. Understanding how histone modifications regulate gene expression will contribute to our understanding of molecular mechanisms underlying the roles played by epigenetic regulation in plants. These studies will provide insights into the gene silencing, DNA methylation and other histone modification-associated gene regulation in plants. Given the importance of epigenetic mechanisms in the regulation of gene activities, a better understanding of how histone modifications modulate plant gene expression in plant stress responses may have wide application to crop improvement.