Liao, Chien-ChiaChien-ChiaLiaoChiu, Chiao-JunoChiao-JunoChiuYAO-HSU YANGBOR-LUEN CHIANG2022-09-162022-09-162022-05-2025890042https://scholars.lib.ntu.edu.tw/handle/123456789/620620Stem/progenitor cells, because of their self-renewal and multiple cell type differentiation abilities, have good potential in regenerative medicine. We previously reported a lung epithelial cell population that expressed the stem cell marker SSEA-1 was abundant in neonatal but scarce in adult mice. In the current study, neonatal and adult mouse-derived pulmonary SSEA-1+ cells were isolated for further characterization. The results showed that neonatal-derived pulmonary SSEA-1+ cells highly expressed lung development-associated genes and had enhanced organoid generation ability compared with the adult cells. Neonatal pulmonary SSEA-1+ cells generated airway-like and alveolar-like organoids, suggesting multilineage cell differentiation ability. Organoid generation of neonatal but not adult pulmonary SSEA-1+ cells was enhanced by fibroblast growth factor 7 (FGF 7). Furthermore, neonatal pulmonary SSEA-1+ cells colonized and developed in decellularized and injured lungs. These results suggest the potential of lung-derived neonatal-stage SSEA-1+ cells with enhanced stem/progenitor activity and shed light on future lung engineering applications.enDevelopmental biology; Stem cells research; Tissue Engineering[SDGs]SDG3journal article10.1016/j.isci.2022.104262355215162-s2.0-85129215715https://scholars.lib.ntu.edu.tw/handle/123456789/611040