2013-11-252024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/660240摘要：國內部份河川汙染嚴重，然而底泥中毒性物質及重金屬之污染程度與其對生態的影響，尚無法正確的評估，尤其是這些重金屬相較於自然產生之重金屬，移動性高，且較易因環境擾動而釋出，直接或間接的影響生態系統甚至擴及地下水。因此，開發快速篩測並降低檢測成本的方法，用以偵測底泥中毒性物質及重金屬移動與地下水毒性物質及重金屬汙染，對於有關單位管理毒性物質及重金屬汙染是非常重要的議題。 因此，本研究計劃建構一系列專一性細菌生物檢測器，用以快速篩測底泥及地下水現場的重金屬。其中包含以綠色螢光蛋白(Green Fluorescent protein, gfp)、lacZ、lux為顯示訊號之細菌生物感測器，探討細菌生物檢測器感測靈敏度與穩定度。我們預計以汞、砷、鎘、鉛等重金屬為專一性標的。另外，因環境中的底泥及地下水可能含有除了重金屬之外的汙染物，因此，本研究亦計劃開發能用於偵測廣效性(非專一性)有毒物質的生物感測器。 本計畫預計完成：(1)底泥與地下水樣本收集，並以化學法分析其重金屬含量；(2)建立廣效性毒性生物感測器之標準檢測方法；(3)建構一系列重金屬專一性生物檢測器，完成其靈敏度與穩定度之探討；(4)比較分析化學法及生物感測器的結果及成本。我們希望藉由這個專案計畫，建立毒性物質及專一性重金屬檢測器組套，提供快速篩測底泥及地下水汙染物的方法並降低檢測成本的方法，作為有關單位對於毒性物質及重金屬環境汙染的有效管理。 <br> Abstract: Certain rivers in Taiwan are heavily polluted. However, the toxicity and ecological impact of pollutants in the sediment are difficult to accurately assess. In particular, heavy metals in the sediments might release to aquatic environment and groundwater directly or indirectly affect ecosystem and human health. Therefore, there is urgent need to develop sensitive and inexpensive methods for rapidly detecting pollutants in the sediments and groundwater. In this grant, we propose to establish a set of specific bacterial biosensors to detect heavy metals (Hg, As, Cd, and Pb) in the sediments and groundwater. Heavy metals-specific bacterial biosensors carrying different reporter gene (gfp, lacZ, and luxCDABE) will be genetically engineered to investigate the sensitivity and stability of bacterial biosensors. In addition, toxic chemicals besides heavy metals are frequently present in the sediments and groundwater, we also propose to develop a toxicity biosensor to detect general pollutants. Thus, the specific objectives of this research grant are: (1) to collect the sediment and groundwater samples and analyze pollutants by chemical methods, (2) to establish the standard methods to examine pollutants /toxicity by using sulfur-oxidizing bacteria (SOB), (3) to construct a series of bacterial biosensors for specific heavy metals (Hg, As, Cd, and Pb), and (4) to compare and analyze the results and cost from chemical methods and bacterial biosensors. We hope results from this study can provide valuable methods and information to rapidly detect pollutants in the sediment and groundwater and incorporate into the management strategies for environmental quality control.細菌生物感測器快速篩測底泥地下水污染物bacterial biosensorrapid detectionsedimentgroundwaterpollutant