2012-08-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/679229摘要：根據國際政府間氣候變遷委員會(IPCC)預期本世紀人類因為地球前所未見的乾旱與高溫將遭逢前所未有的糧食危機。環境逆境造成世界上農業方面廣泛的損失。個別的生物逆境，例如:乾旱、鹽、高熱、採收後老化與病害都受到熱烈的研究。雖然過去20年來已經累積了大量的知識，但是在阿拉伯芥仍然有許許多多對逆境反應的基因沒有被研究過。在本團隊計劃中，我們將對非生物性逆境與生物性逆境相關的基因或蛋白質進行基礎的研究。在子計劃當中，有二個將探討乾旱逆境(子計劃:1和6)，兩個與鹽逆境有關(子計劃2和6)，兩個與熱逆境有關(子計劃3和4)，二個與病毒逆境有關(子計劃8和9)，另外各有一個與老化與光逆境相關。我們將使用功能性基因體，蛋白質體，結構生物資訊，分子遺傳與植物生理的方法來研究這些特殊基因的功能。九個子計劃所用的研究方法實際上是互補的。我們所列舉研究的基因均有初步的結果並且均在逆境方面扮演調節之功能。這些基因包括了lectin receptor kinases (LecRKs), histone deacetylase 15 (HDA15), LSD1-like histone demethylase (LDLs); 鈣離子結合蛋白基因, like OsCaM1-1; 轉錄子如, AtDS1, Aintegumenta-Like 7 (AIL7), AtWRKY70, AtWRKY54, S1 class bZIP11, 尚有其他未知功能的基因如，miRNAs, C3HC4-type RING E3 ligase (SlREL1), RNase L inhibitor (SlRLI), neutral invertase (AtCINV1), drought inducible protein 19-2 (AtDi19-2). 這些都會加強子計劃之間的凝聚力與合作關係。雖然我們均進行獨立研究，卻也發展子計畫之間密切交互作用來解決生物研究上的問題，這將強化我們在植物逆境基礎研究方面的成果，並且對未來作物改良有所貢獻。<br> Abstract: Future food security is under serious threat because unprecedented heat and drought in this century were forseen by the the Intergovernmental Panel on Climate Change (IPCC). Environmental stress conditions cause extensive losses to agricultural production worldwide. Individually, stress conditions such as drought, salinity, heat, post-harvest senescence and microorganisms have been the subject of intense research. Though a great deal of knowledge has been advanced in the past 20 years, a large array of genes responsive to stresses in Arabidopsis is still unknown and unstudied. In this team project we would like to study the basic aspect of abiotic and biotic stresses related genes and proteins. Two projects deal with the dehydration stress (sub-projects 1 and 6), two with salt stress (sub-projects 2 and 6), two with heat stress (sub-projects 3 and 4), two with pathogen stress (sub-projects 8 and 9) and one each with senescence and light stress. We will use functional genomics, proteomics, structure bioinformatics, molecular genetics and physiological approach to study the specific gene function. The approaches used by all sub-projects are complementary. All the genes will be explored in this team project have some preliminary results and play regulatory role in stress responses. These includes upstream regulatory genes, like lectin receptor kinases (LecRKs), histone deacetylase 15 (HDA15), LSD1-like histone demethylase (LDLs); calcium binding protein, like OsCaM1-1; transcription factors like, AtDS1, Aintegumenta-Like 7 (AIL7), AtWRKY70, AtWRKY 54, and S1 class bZIP11, and other unknown function genes like, miRNAs, C3HC4-type RING E3 ligase (SlREL1), an RNase L inhibitor (SlRLI), a, neutral invertase (AtCINV1), drought inducible protein 19-2 (AtDi19-2). This will enhance coherence and cooperation among sub-projects for the common interest. While carrying out the research independently, in fact, developing strong interactions among sub-projects or laboratories working on a defined biological problem will strengthen our basic research and has a potential to contribute to agriculture improvement.阿拉伯芥轉錄子Aintegumenta-Like 7AtDS1乾旱誘導負向調控者Arabidopsis thalianaAintegumenta-Like 7AtDS1ERF/AP2 transcription factornegative regulator