2009-06-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/659273摘要：收集本省各地區之養殖正常和病牡蠣，針對熱休克蛋白以分子生物技術包括引子設計，聚合連鎖反應，質體轉型，兔抗牡蠣熱休克蛋白質多株抗體血清製備，酵素連結免疫吸附法來檢查熱休克蛋白含量和嘉義雲林沿海養殖牡蠣之死亡間關係，從而研發出以熱休克蛋白含量作為牡蠣受緊迫之指標，在環境中水質因子等改變或病原入侵早期即可預防。其經濟效益： (1)檢查及確認感染養殖牡蠣之疾病，研發出以熱休克蛋白含量作為牡蠣受緊迫之指標，在環境中水質因子等改變或病原入侵早期即可預防。早期發現疾病 預期成果:本年度 40% (2)建立檢驗緊迫之指標，期能快速發現疾病 預期成果:本年度 35% (3)增加防疫機關之防疫能力 預期成果:本年度 25% 2. 其他政策效益或不可量化效益： 依漁民需求組成研究調查小組協助漁民調查，針對牡蠣養殖過程中之緊迫包括環境中水質因子等改變或病原入侵早期即可預防疾病等，協助漁民了解牡蠣死亡原因。<br> Abstract: Mortality of cultured oyster has been reported in Southern Taiwan without any pathogens were deterimened. In the epizootic, Rickettsia-like organisms were detected in the mantle, gill, and visceral organs of moribund oyster. However, further examination of cultured oyster in other locations without mortality has also detected these Rickettsia-like organisms in the mantle, gill, and visceral organs, although their quanitity was less prominent than those of moribund oyster in the epizootic. This finding has impress us that Rickettsia-like organism may act as a indicator of stress in this epizootic. In order to evaluate the relationship between the stress response of oyster, heat shock protein (HSP) concentration in the animal may be a good indicator of stress. In the study, primer sets associated with HSP will be designed to prepare rabbit anti-HSP antibody and apply to the detection of HSP concentration in the tissues and hemolymph of oyster using ELISA technique.牡蠣熱休克蛋白緊迫oysterheat shock proteinstress