Tsai W.-B.Ng S.C.Bernotiene E.Sobolev A.2019-05-282019-05-282019978151088390115267547https://scholars.lib.ntu.edu.tw/handle/123456789/410731Statement of Purpose: Hydrogels have been popularly used as scaffolds in tissue engineering. Particularly, in situ gelling systems have drawn a lot of attention due to the elimination of open surgical process and the ease of administration. In the study, an in situ gelling system that based on the ECM proteins and glycosaminoglycans (GAG). Phenolic or catecholic groups could be crosslinked via enzymatic reaction of horse radish peroxidase (HRP) and laccase. The mechanism, the induction of oxidative coupling of the phenolic moieties by HRP/H2O2, is inspired by the natural process of melanin formation. The conjugation of tyramine to GAG could be easily fulfilled by a general carbodiimide/active ester-mediated coupling reaction. Proteins, such as gelatin and collagen, containing tyrosine residues could also form hydrogels via the same enzymatic mechanism. The goal of this study is to develop a enzymatic gelling hydrogel composed of GAG/ECM proteins for encapsulation of mesenchymal stem cells in tissue engineering applications. ? 2019 Omnipress - All rights reserved.conference paper2-s2.0-85065432478https://www.scopus.com/inward/record.uri?eid=2-s2.0-85065432478&partnerID=40&md5=0f3e85b02e70388273789bf24b97124e