2010-03-082024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/683866摘要：臺灣得天獨厚的地理環境，蘊育了許多深受世界各國研究人員注目之珍貴數種。獨特的樹種中往往具有一些特殊成分。因此，若能自本土樹木中尋求具生物活性之成分，鑑定其結構並釐清其作用之機制，將可賦予森林利用新的價值與潛能。本計畫擬選擇臺灣特產並具研究潛力之五加科木本植物，進行包括抗腫瘤、抗發炎和代謝症候群治療等生物活性之篩選。並利用管柱層析、氣相層析及高效能液相層析等技術，分離、純化並收集具生物活性之成分。之後，續運用質譜分析，紅外光光譜分析，碳譜與氫譜核磁共振光譜分析等光譜分析，鑑定具生物活性成分之確切結構。此外並將就分子生物層面，解析這些特殊成分之生合成。本計畫如能順利完成，將有助於擴展本土五加科木本植物加工利用之潛能與應用領域。<br> Abstract: Being richly endowed by nature, there are many precious tree species grown in Taiwan. Different wood species and even various parts of the same tree, such as stem, leaves, and bark, vary distinguishably with respect to both amount and chemical compositions of extractives. Therefore, to identify the bioactive components from the diversified wood extractives and to clarify the mechanisms of their bioactive functions could provide an opportunity to expand the potential utilization of tree. In this purposed project, the bioactive compounds that isolated from the extractives of the endemic Araliaceae plants in Taiwan, will be examined. The bioactivities investigated include antitumours, antiinflammation, and Symdron X therapy. Repeatedly through the chromatography separation, spectral analysis, and bioactive assay, the bioactive compounds of Araliaceae plants indigenous to Taiwan will be confirmed. In addition, the molecular biology techniques will be performed to study the genomic diversity of the genes, which involved in the biosynthesis of bioactivity compounds. These will obtain the genes associated with metabolism to provide the useful information for the researches on tree physiology, evolution, gene transformation, and drug discovery in the future.五加科細胞毒性生合成AraliaceaecytotoxicityBiosynthesis