Yang Y.-C.Lin H.-Y.KANG-YI SUChen C.-H.Yu Y.-L.Lin C.-C.SUNG-LIANG YUYan H.-Y.Su K.-J.Chen Y.-L.S.2020-06-302020-06-3020121741-427Xhttps://scholars.lib.ntu.edu.tw/handle/123456789/507252Saussurea involucrata (Kar. et Kir.), known as the snow lotus, grows in the Tian Shan and A'er Tai areas of China. It has recently been reported that the ethyl acetate extract of S. involucrata (SI-2) can inhibit proliferation and induce apoptosis in PC-3 human prostate cancer cells. This study investigated the protective effect of ethyl acetate extract of S. involucrata (SI-2) or rutin, a flavonoid extracted from ethyl acetate extract of S. involucrata (SI-2), on D-galactose- (D-gal-) induced brain injury in mice. Administering SI-2 or rutin (30 mg/kg/d and 30 mg/kg/d) for 6 weeks, concomitant with D-gal injection, significantly increased superoxide dismutase and glutathione peroxidase activities and decreased the MDA level in plasma. Furthermore, the result showed that the percentages of cleaved caspase-3 and PARP in the D-gal-treated mice were much higher than those in the control. Pretreatment using SI-2 or rutin decreased the expression of cyclooxygenase-2 via downregulation of NF-kappaB, resulting in a decrease in lipid peroxidation. Furthermore, our results also showed that oral administration of rutin to these mice significantly improved behavioral performance in a step-through passive avoidance task and these results suggest that SI-2 or rutin exerts potent antiaging effects on D-gal in mice via antioxidative mechanisms. ? 2012 Ying-Chen Yang et al.[SDGs]SDG3caspase 3; cyclooxygenase 2; galactose; glutathione peroxidase; immunoglobulin enhancer binding protein; lipid; malonaldehyde; nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase; rutoside; superoxide dismutase; animal experiment; animal model; animal tissue; antioxidant activity; article; brain injury; controlled study; drug structure; enzyme activity; lipid peroxidation; male; medicinal plant; mouse; nonhuman; priority journal; protein cleavage; protein expression; receptor down regulation; Saussurea involucrata; senescencejournal article10.1155/2012/9802762-s2.0-84866142918