You, Y.-H.Y.-H.YouLin, Y.-F.Y.-F.LinNirosha, B.B.NiroshaChang, H.-T.H.-T.ChangHuang, Y.-F.Y.-F.Huang2019-07-312019-07-312019https://www.scopus.com/inward/record.uri?eid=2-s2.0-85069262237&doi=10.7150%2fntno.36842&partnerID=40&md5=32b2a720eb644787ae6f1216d98e0ba9https://scholars.lib.ntu.edu.tw/handle/123456789/415873Cancer combination therapy can improve treatment efficacy and is widely utilized in the biomedical field. In this paper, we propose a facile strategy to develop a polydopamine (PDA)-coated Au nanostar (NS@PPFA) as a multifunctional nanoplatform for cancer targeting and combination therapy. The Au nanostar demonstrated high photothermal conversion efficiency because of the tip-enhanced plasmonic effect. Modification of PDA and folic acid on the NS surface improved its drug-loading efficiency and targeting capability. In vitro, compared with nontargeted cells, targeted breast cancer MCF-7 cells demonstrated efficient uptake of chemodrug-loaded NS-D@PPFA through the receptor-mediated endocytosis pathway. In combination with the photothermal effect induced by near-infrared laser irradiation, controlled payload release could be activated in response to both internal (acid) and external (photothermal) stimuli, leading to an efficient chemo-photothermal action against MCF-7 cells and drug-resistant MCF-7/ADR cells. By contrast, cellular damage was less obvious in normal HaCaT (human skin keratinocytes) and NIH-3T3 cells (murine fibroblasts). In addition, payload-free NS@PPFA exhibited a high binding affinity (Kd = 2.68 × 10-10 M) toward vascular endothelial growth factor (VEGF-A165), which was at least two orders of magnitude stronger than that of highly abundant plasma proteins, such as human serum albumin. Furthermore, in vitro study showed that NS@PPFA could effectively inhibit VEGF-A165-induced proliferation, migration, and tube formation of human umbilical vein endothelial cells, resulting in additional therapeutic benefits for eradicating tumors through a simultaneous antiangiogenic action in chemo-photothermal treatment. The combined treatment also exhibited the lowest microvessel density, leading to a potent antitumor effect in vivo. Overall, our "all-in-one" nanoplatform is highly promising for tumor therapy, enabling effective treatment against multidrug-resistant cancers.Antiangiogenesis; Cancer combination therapy; Drug delivery; Gold nanostar; Multidrug resistance; Polydopamine[SDGs]SDG3angiogenesis inhibitor; antineoplastic agent; gold; indole derivative; nanoparticle; polydopamine; polymer; vasculotropin A; angiogenesis; animal; apoptosis; breast tumor; cell motion; cell proliferation; chemistry; drug effect; drug release; drug resistance; endocytosis; female; human; MCF-7 cell line; metabolism; mouse; multidrug resistance; NIH 3T3 cell line; nude mouse; phototherapy; temperature; thermotherapy; time factor; tissue distribution; ultrastructure; umbilical vein endothelial cell; Angiogenesis Inhibitors; Animals; Antineoplastic Agents; Apoptosis; Breast Neoplasms; Cell Movement; Cell Proliferation; Drug Liberation; Drug Resistance, Multiple; Drug Resistance, Neoplasm; Endocytosis; Female; Gold; Human Umbilical Vein Endothelial Cells; Humans; Hyperthermia, Induced; Indoles; MCF-7 Cells; Mice; Mice, Nude; Nanoparticles; Neovascularization, Physiologic; NIH 3T3 Cells; Phototherapy; Polymers; Temperature; Time Factors; Tissue Distribution; Vascular Endothelial Growth Factor Ajournal article10.7150/ntno.368422-s2.0-85069262237