Tamm-Horsfall glycoprotein enhances PMN phagocytosis by binding to cell surface-expressed lactoferrin and cathepsin G that activates MAP kinase pathway

Siao, Syue-CianSyue-CianSiaoKO-JEN LISONG-CHOU HSIEHCHENG-HAN WULu, Ming-ChiMing-ChiLuTsai, Chang-YouhChang-YouhTsaiCHIA-LI YU2022-02-102022-02-102011-03-031420-3049https://scholars.lib.ntu.edu.tw/handle/123456789/593994The molecular basis of polymorphonuclear neutrophil (PMN) phagocytosis-enhancing activity (PEA) by human purified urinary Tamm-Horsfall glyco- protein (THP) has not been elucidated. In this study, we found human THP bound to lactoferrin (LF) and cathepsin G (CG) expressed on the surface of PMN, identified by a proteomic study with MALDI-TOF- LC/LC/mass spectrometric analysis. Pre-incubation of 10% SDS-PAGE electrophoresed PMN lysates with monoclonal anti-LF or anti-CG antibody reduced the binding with THP. To elucidate the signaling pathway of THP on PMN activation, we found THP enhanced ERK1/2 phosphorylation, reduced p38 MAP kinase phosphorylation, but had no effect on DNA binding of the five NF-kB family members in PMN. To further clarify whether the carbohydrate-side chains or protein-core structure in THP molecule is responsible for THP-PEA, THP was cleaved by different degrading enzymes with carbohydrate specificity (neuraminidase and β-galactosidase), protein specificity (V8 protease and proteinase K) or glycoconjugate specificity (carboxylpeptidase Y and O-sialoglycoprotein endopeptidase). We clearly demonstrated that the intact protein-core structure in THP molecule was more important for THP-PEA than carbohydrate-side chains. Putting these results together, we conclude that THP adheres to surface-expressed LF and CG on PMN and transduces signaling via the MAP kinase pathway to enhance PMN phagocytosis.enTamm-Horsfall glycoprotein; neutrophil phagocytosis-enhancing activity; lactoferrin; cathepsin G; protein-core structure; carbohydrate-side chain; URINARY GLYCOPROTEIN; HUMAN NEUTROPHILS; PROTEIN BINDS; UROMODULIN; RECEPTORS; RELEASE; LECTIN; OLIGOSACCHARIDES; GRANULOCYTES; SPECIFICITYTamm-Horsfall glycoprotein enhances PMN phagocytosis by binding to cell surface-expressed lactoferrin and cathepsin G that activates MAP kinase pathwayjournal article10.3390/molecules16032119213727592-s2.0-79953078890WOS:000288853400015https://scholars.lib.ntu.edu.tw/handle/123456789/540836