Huang, Jhen-GangJhen-GangHuangLee, Chen-LungChen-LungLeeSHI-MING LINChuang, Tsung-LiangTsung-LiangChuangWang, Way-SeenWay-SeenWangJuang, Rong-HueyRong-HueyJuangWang, Ching-HoChing-HoWangCHIH-KUNG LEECHII-WANN LIN2009-02-042018-06-292009-02-042018-06-29200609565663http://ntur.lib.ntu.edu.tw//handle/246246/120159http://ntur.lib.ntu.edu.tw/bitstream/246246/120159/1/48.pdfhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-33749257088&doi=10.1016%2fj.bios.2006.07.030&partnerID=40&md5=f48c7a599e3d5f670e36c91da23b92fbA surface plasmon resonance (SPR) waveguide immunosensor fabricated by germanium-doped silicon dioxide was investigated in this study. The designed waveguide sensor consisted of a 10 μm SiO2 substrate layer (n = 1.469), a 10 μm Ge-SiO2 channel guide (n = 1.492) and a 50 nm gold film layer for immobilization of biomolecules and SPR signal detection. The resultant spectral signal was measured by a portable spectrophotometer, where the sensor was aligned by a custom-designed micro-positioner. The results of the glycerol calibration standards showed that the resonance wavelength shifted from 628 to 758 nm due to changes of refractive index from 1.36 to 1.418. Flow-through immunoassay on waveguide sensors also showed the interactions of protein A, monoclonal antibody (mAb ALV-J) and avian leucosis virus (ALVs) resulted in wavelength shifting of 4.17, 3.03 and 2.18 nm, respectively. The SPR dynamic interaction could also be demonstrated successfully in 4 min as the sensor was integrated with a lateral flow nitrocellulose strip. These results suggest that SPR detection could be carried out on designed waveguide sensor, and the integration of nitrocellulose strip for sample filtering and fluid carrier would facilitate applications in point-of-care portable system. © 2006 Elsevier B.V. All rights reserved.application/pdf751563 bytesapplication/pdfen-USImmunosensor; Surface plasmon resonance; WaveguideDoping (additives); Germanium; Silica; Spectrophotometers; Surface plasmon resonance; Waveguides; Avian leucosis virus (ALV); Fluid carriers; Point-of-care portable systems; Waveguide sensors; Immunosensors; germanium; silicon dioxide; analytic method; article; calibration; coated particle; electroimmunoassay; immobilization; material coating; optical biosensor; point of care testing; refraction index; signal detection; surface plasmon resonance; Avian leukosis virusjournal article10.1016/j.bios.2006.07.030169627632-s2.0-33749257088http://ntur.lib.ntu.edu.tw/bitstream/246246/120159/1/48.pdf