2019-01-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/672131摘要：隨著高科技產業蓬勃發展所產生含有鎵、銦及鉈等新興汙染元素的放流水若進入農業灌溉系統，可能會因干擾農地中土壤微生物活性。由於土壤微生物多樣性不僅對於土壤構造形成和地化循環正常運行不可或缺，在決定土壤肥力、植物生長狀況及其耐逆境能力上，更是扮演重要角色。因此若土壤微生物活性受影響，可能會對整個土壤-糧食作物系統造成極大衝擊。因此，本計畫擬透過 16S rRNA 基因定序方法及總體基因體學等方法，分析臺灣代表性土壤受鎵、銦及鉈污染前後微生物相的變化，以了解新興污染元素是否會對土壤微生物族群組成或功能造成影響。此外，亦會探索對新興污染元素具抗性的菌株，瞭解其特性與評估未來應用於生物復育之潛力。 <br> Abstract: With the flourish high-tech industries, the activities of soil microorganisms in the agricultural fields might be interfered if effluent containing emerging contamination elements (ECEs), such as gallium (Ga), in (I) and tallium (Ta), is released into irrigation systems. Soil microbial diversity not only is required for soil formation and geochemical cycles, but also plays important roles in determination of soil fertility, plant growth and plant resistance to stresses. If ECEs would affect activities of soil microorganisms, they could cause great impacts on the whole soil-food system. Therefore, changes of soil microbiomes in the representative soil in the presence of ECEs will be analyzed using 16S rDNA sequencing and shotgun metagenomics to assess the effect of ECEs on soil microbiomes. In addition, microbial isolates resistant to ECEs will be explored to understand their characteristics and potentials to be used for bioremediation of ECEs-contaminated soil in the future新興污染元素次世代定序16S rRNA 基因序列總體基因體學微生物相Emerging contamination elementsnext generation sequencing16S rRNA gene sequencingmetagenomicsmicrobiome