蔡懷楨臺灣大學:分子與細胞生物學研究所凃啟堂Tu, Chi-TangChi-TangTu2010-06-022018-07-062010-06-022018-07-062009U0001-2807200922173700http://ntur.lib.ntu.edu.tw//handle/246246/184752Although the embryonic expression pattern of ADP ribosylation factor-like 6 interacting protein 1(Arl6ip1) has been reported, the function that Alr6ip1 plays ineural crest development is unclear. We found that knockdown of Arl6ip1 caused defective neural crest derivatives in embryos, particularly more severe in craniofacial cartilages. Expressions of neural plate border specifier genes msxb, dlx3b, and pax3 were normal, but the expressions of the neural crest specifier genes foxd3, snai1b, and sox10 were greatly reduced. These findings suggest that arl6ip1 is essential for neural crest specification, but not neural crest induction. Furthermore, we revealed that the streams of crestin- and sox10-expressing neural crest cells which migrate ventrally fromeural tube into trunk were disrupted in arl6ip1 morphants. This migration defect was not only in the trunk neural crest, but also in the enteric tract where the vagal-derivedeural crest cells failed to populate the enteric nervous system. These data further suggested that arl6ip1 is required for neural crest migration. Finally, we proved thatpoptosis is not attributed to the loss of neural crest cells , confirmed by double staining of TUNEL and crestin. Therefore, we concluded that arl6ip1 is required for neural crest specification and migration.中文摘要 ------------------------------------- 1bstract ------------------------------------- 2ntroduction ----------------------------------3aterials and Methods -------------------------8esults ---------------------------------------12iscussion ------------------------------------23eferences ------------------------------------28igures and supplementary figures -------------33able -----------------------------------------46ublication -----------------------------------48application/pdf9627475 bytesapplication/pdfen-US神經脊特化因子斑馬魚神經脊發育neural crest specifierzebrafishArl6ip1neural crest developmenthttp://ntur.lib.ntu.edu.tw/bitstream/246246/184752/1/ntu-98-F90243002-1.pdf