2012-06-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/656511摘要：活髓治療發展至今在臨床上仍然沒有理想之材料。本計畫目的為改良磷酸鈣骨水泥(CPCs)生醫陶瓷材料應用於活髓治療，藉由結合和康生物科技公司原有的產業優勢及生醫材料研發的技術，合作研發改良適合活髓治療可引導牙髓-骨質再生之新型磷酸鈣(MBI-CPCs)複合生醫陶瓷材料，並調控磷酸鈣生醫陶瓷之初期強度與硬化時間，應用於引導牙髓與骨再生之治療，解決現今臨床材料的限制與缺點。本計畫預計分三年執行，第一年研發製程：以和康公司研發之新型磷酸鈣骨水泥(MBI-CPCs)與藉由功能性高分子(聚麩胺酸-明膠)添加調控材料性質，分析產物的物理化學性質及細胞毒性，篩選製程穩定、結構理想且性質差異具代表性的材料進入第二年實驗。第二年：利用該材料組成及性質的高調控性，進行細胞行為調控的研究，藉以尋找牙髓牙本質修復再生所需的環境因素及機制與引導骨再生的相關機制，並分析材料生物相容性，篩選出生物相容性高的產物。第三年:探討各種材料之物理化學性質、生物相容性、活體外誘導牙髓細胞分化能力等變因，是否在動物活髓治療模型確實可以誘導牙本質牙髓組織再生，驗證第二年研究結果所闡釋的誘導牙本質牙髓組織再生的調控機轉。期望經由以上之整體規劃，研發合適材料作為理想活髓之治療，可作為臨床前測試並提供日後醫學轉譯研發新型生醫活性材料之先導與進入產業開發相關醫療產品之先驅。<br> Abstract: Pulp vitality is extremely important for the tooth viability, since it provides nutrition and acts asbiosensor to detect pathogenic stimuli. Vital pulp therapy is a treatment modality with less traumaand minimum invasion for dental pulp diseases. There has not been an ideal material developedfor vital pulp therapy in the past decade. Calcium phosphate cements (CPCs) are an interestingclass of bone substitute materials. However, poor handling property, low material strength and longsetting time are their main problems for vital pulp therapy. In this industry-university cooperation,our groups cooperate with Maxigen Biotech Inc. to develop novel combination bioceramics forpulp-dentin regeneration.The purpose of this study will investigate the effects of modified CPCs on the mechanicalproperties, growth, and odontoblastic differentiation in dental pulp cells, animal study comparedwith clinical materials such as calcium hydroxide and mineral trioxide aggregate (MTA).This project has been arranged for three years, and the specific aims are as follows:First year:(1) Analyze the physico-mechanical properties and biocompatibility of novel combinationbioceramics.(2) Cytotoxicity studies with MTT, LDH and Agar overlay assay will be conducted.(3) Investigate proliferation, mineralization, and differentiation of dental pulp cells on variablebiomaterials.Second year:(1) Establish an in vitro model using dental pulp cells for materials induced dentinogenesis.(2) Evaluate variable bioactivities of novel combination bioceramics、Ca(OH)2 and MTA.(3) Investigate the reguratory mechanisms of materials induced dentiongenesis by proteomics.Third year:(1) Evaluate the effects of novel combination bioceramics on dentin-pulp complex regeneration inanimal model.(2) Compare the results of in vivo animal model with the bioactivity results from in vitro model inthe preceding year.磷酸鈣骨水泥活髓治療牙髓牙本質再生生物相容性calcium phosphate cementvital pulp therapypulp-dentin regenerationbiocompatibility