2012-08-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/664616摘要：研究生物化學的領域中，多數分子結構與特性的量測必須在水溶液環境中，由於原子力顯微術相對於掃描穿隧電子顯微術、電子顯微術等顯微技術，在於成像環境與樣品製備方面的限制較少，因此在水溶液環境之量測經常仰賴原子力顯微術，然而在水溶液中擷取高解析度影像的關鍵問題在於提高訊噪比與力量靈敏度。原子力顯微鏡的輕敲模式利用壓電元件激振微懸臂探針產生共振，此模式可減小對樣品的損壞，當原子力顯微鏡的微懸臂探針浸入水溶液中，水溶液的黏滯性大幅降低振動的Q值(Quality factor)，更是阻礙共振模態的激發；外界環境干擾同時也容易透過探針載台以及水溶液介質傳遞至微懸臂探針，進而影響成像品質。本計畫研究目的在於研究開發適用於水溶液環境的微懸臂探針與探針載台，藉由不同造型的微懸臂探針，以減少水溶液介質阻尼的影響，對探針夾持預壓力進行定量分析，設計合適的探針夾持方式、探針載台材料與幾何結構，以達到降低外界干擾的影響，同時提高探針共振模態的振動強度，進而提高原子力顯微鏡在水溶液中的解析度。<br> Abstract: In the research of biochemistry, most measurements of the molecular structures and properties are operated in the liquid environment for approximating the physiological state. Comparing with scanning tunneling microscopy (STM) and scanning electron microscopy (SEM), atomic force microscopy (AFM) has less restriction on the sample preparation and environment, and is utilized for liquid environment widely. In the tapping mode AFM, the cantilever tip is vibrated on the resonant frequency for avoiding sample damage. However, the Quality factor of the cantilever reduces significantly due to the high viscosity in liquid. Besides, the external disturbance can transmit to the cantilever through the liquid medium easily. Therefore, reducing the liquid damping effect and disturbance are two important issues. In this project, different shapes of microcantilever tip will be designed for reducing fluid damping influence. On the other hand, the cantilever material and geometry can be optimized for minimizing the external disturbance. Besides, we plan to develop a tip holder with an innovative mechanism of cantilever clamping, and analyze the relation between the clamping force and cantilever resonant intensity.原子力顯微鏡水溶液環境懸臂探針探針載台Atomic force microscopeLiquid environmentMicrocantilever tipTip holder