2007-01-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/682520摘要：東方果實蠅為熱帶地區極為重要之果樹害蟲，其危害的栽培作物全世界則超過150種，在台灣則約有50餘種。東方果實蠅的防治以誘殺為主，最早的誘引劑為甲基丁香油所製成的雄性誘引劑，另外，也逐漸發展出以蛋白質水解物為主的食物誘引劑。除上述的兩種誘引劑之外，產卵誘引劑乃是最直觀，且深具研發潛力的防治方式，直接誘引並殺死雌蟲，具有專一性，避免殺死非標的昆蟲。本研究即是希望藉由化學分析與神經生理的技術，研究東方果實蠅的寄主植物-番石榴，與具有誘引效果非寄主植物-爪哇鳳果，探討雌蟲尋找寄主的化學物質，以開發出更安全、有效之誘引劑，減輕東方果實蠅所造成之經濟危害。本研究藉由收集植物的揮發性物質，經由GC-MS鑑定成分，並透過GC-EAD測試東方果實蠅的對化學物質的神經反應，並透過行為實驗，測定具有誘引力的化學物質。 採用誘引劑作為東方果實蠅防治措施，不僅可減少農藥的使用並消彌農藥殘留的問題，也降低對於周遭生態環境的衝擊。誘雌化學物質的開發將比目前所使用的甲基丁香油的誘雄藥劑更直接的增強防治效果，同時也可應用於田間族群監測上，與甲基丁香油一併使用。 <br> Abstract: The oriental fruit fly is the most singnificant fruit pest in the tropical area. The host range is more than 150 species over the world, and more than 50 species in Taiwan. The main method of oriental fruit fly managemet is the earlist male attractant made by methyl eugenol. Besides, protein bait is developed as a food attractant. In addition to these two attractants, female attractant is the method with the most direct and study potential. It is species specific to attract and capture females, but avoiding killing non-target insects. This project researches the volatile of the host plant-guava and the non-host plant-Garcinia dulcis by combining chemical analyzing and neurophysiology techniques. In order to find out the chemical that female locates host plants, safer and more effective attractant is developed for reduceing the economic loss caused by the oriental fruit fly.This study tests the attractive chemicals by four steps: first, collecting the plant votalite; second, identified by GC-MS ; third, testing the neuron reaction by GC-EAD; last, behavioral test. Controling the oriental fruit fly by attractants is not only reducing the usage of pesticides and avoiding the problem of pesticide residue but also decreasing the ecological impact of local environment. Comparing to the methyl eugenol, the female attractant is more efficient in controlling. Both of methyl eugenol and the female attractant could be exercised in the population detection simultaneously.東方果實蠅化學訊息物氣相層析-觸角電圖Bactrocera dorsalissemiochemicalGC-EAD