Wu C.-C.Chu Y.-L.Sheen L.-Y.2019-07-112019-07-11201222254110https://scholars.lib.ntu.edu.tw/handle/123456789/413562The effect of allicin, an active ingredient of garlic, on lactate dehydrogenase (LDH) leakage, lipid peroxidation, glutathione (GSH) content, and GSH-related enzyme activity was investigated in primary hepatocytes. In this study, allicin was synthesized in our laboratory as an experimental material, and primary hepatocytes isolated from Sprague-Dawley rats were used as an experimental model. According to the results, hepatocytes treated with 10 £gM allicin did not differ from the control on LDH leakage during various incubation times. When the hepatocytes were treated with 10 £gM allicin, their levels of thiobarbituric acid reactive-substances (TBARS) did not differ significantly from that of the control within the 8-h incubation. However, the TBARS values of hepatocytes treated with 30 and 50 £gM allicin were higher compared to the control after incubation for 4 h and 8 h, respectively. The hepatocyte intracellular GSH content was significantly higher than that of the control after 30 £gM allicin treatment, but treatment with 50 £gM allicin caused a significant GSH depletion after incubation for 4 h or longer. In addition, when hepatocytes were treated for 24 h with 10 or 30 £gM allicin, glutathione peroxidase (GPx) activity was significantly increased compared to that of the control, whereas 50 £gM allicin treatment for 24 h or longer significantly decreased the GPx activity. Glutathione reductase (GRd) activity was significantly increased when the hepatocytes were treated with 10 £gM allicin for 24 h, but GRd activity significantly decreased when the hepatocytes were treated with 50 £gM allicin. However, hepatocytes treated for 24 h with 10 or 30 £gM allicin showed significantly increased glutathione S-transferase (GST) activity compared to the control. These results suggest that 10 £gM allicin potentially enhances the antioxidation and detoxification capabilities of primary rat hepatocytes. Copyright ? 2011 Committee on Chinese Medicine and Pharmacy, Taiwan.AllicinAntioxidationDetoxificationPrimary rat hepatocytesjournal article10.1016/S2225-4110(16)30118-3https://www.scopus.com/inward/record.uri?eid=2-s2.0-84894590702&doi=10.1016%2fS2225-4110%2816%2930118-3&partnerID=40&md5=3187d00605e4bf0271e9b513ce6b568c2-s2.0-84894590702https://www.scopus.com/inward/record.uri?eid=2-s2.0-84894590702&doi=10.1016%2fS2225-4110%2816%2930118-3&partnerID=40&md5=3187d00605e4bf0271e9b513ce6b568c