外科CHUNG, TZE-WENTZE-WENCHUNGWANG, SHOEI-SHENSHOEI-SHENWANG2010-06-232018-07-112010-06-232018-07-112009http://ntur.lib.ntu.edu.tw//handle/246246/185877The bioactivity of poly (epsilon-caprolactone) (PCL) films is improved by grafting chitosan (CS) surfaces with various values of nano-roughness on PCL surfaces. To examine the effects of the design, growing human gingival fibroblasts HGFs) on the films was conducted. Various values of nano- rough CS surfaces were cast using nano-rough PCL molds that had been fabricated using a solvent-etched technique. The features of nano-CS/PCL surfaces were characterized using an atomic force microscope (AFM) to observe the topography and to determine the value of centerline average roughness of a surface, R-a. The R-a values of the nano-CS/PCL films were 36.8 +/- A 1.6, 100.0 +/- A 3.0, and 148 +/- A 7.0 nm, while that of the smooth CS/PCL film was 12.5 +/- A 1.6 nm. The growth and proliferation of HGFs on the films are elucidated by fluorescent staining and analyzed by MTT viability assay following three and 7 days of culture. The viability assay of the cells reveals that the growth rates of HGFs on both CS/PCL and nano-CS/PCL films significantly exceed (95% or more; P < 0.001) those of PCL on both days, demonstrating the improvement of the bioactivity of PCL films by grafting CS. Additionally, the growth rates and proliferations of HGFs on nano-CS/PCL films of roughness 100 and 148 nm markedly exceed (15% or more; P < 0.001) those on 36.8 nm nano-CS/PCL and CS/PCL films, after both periods of culturing, indicating that the high nano-roughness CS surfaces further enhance the growth rate of HGFs. In conclusion, markedly improving the bioactivity of PCL films by grafting CS is demonstrated. Moreover, high nano- roughness of nano-CS/PCL films can further accelerate the growth and proliferation of HGFs compared with those of CS/ PCL films. This work presents a new concept for designing biomaterials in tissue engineering.en-USjournal article