Zhuang, Yi RuYi RuZhuangYUAN-YU LIN2023-03-132023-03-132022-01-0115163598https://scholars.lib.ntu.edu.tw/handle/123456789/629236The objective of this study was to investigate the effects of recombinant adiponectin on chicken liver cells. The full-length chicken adiponectin gene was amplified by PCR and cloned into the vector pET-32a, followed by the transformation of the vector into Escherichia coli BL21. SDS-PAGE was used to detect and analyze the purity of the expressed recombinant protein. Induction was performed with 1 mM IPTG at 30 °C for 3 h, and the recombinant thioredoxin–adiponectin fusion protein was purified using Ni-NTA affinity chromatography. Chicken adiponectin was successfully expressed and purified in a bacterial system. In addition, the chicken recombinant adiponectin demonstrated that it ameliorates palmitic acid and oleic acid-induced adipogenesis, in which an increase in β-oxidation and a decrease in lipogenesis-related genes may be involved. In summary, chicken recombinant adiponectin enhances fatty acid metabolism in LMH cells.chicken | fatty acid metabolism | recombinant proteinjournal article10.37496/RBZ51202200872-s2.0-85148630934https://api.elsevier.com/content/abstract/scopus_id/85148630934