Tsai M.-S.WEN-HSI LINWEN-MING HSUHONG-SHIEE LAICHI-CHUAN YEHJOHN HUANGHAO-CHIH TAIPO-HUANG LEEChen W.-J.2020-11-192020-11-1920100022-4804https://www.scopus.com/inward/record.uri?eid=2-s2.0-78449273245&doi=10.1016%2fj.jss.2009.05.040&partnerID=40&md5=370afde0c74f41e9ea3738b29168d8b0https://scholars.lib.ntu.edu.tw/handle/123456789/521305Background: Intestinal transplantation (ITx) is a life-saving procedure for patients with intestinal failure. The poorer outcome of ITx than of other organ transplantation, however, warrants more clinical and basic research on ITx. Herein, we developed a modified Paul-Mikulicz ileostomy procedure in a swine model of ITx, and investigated its feasibility for obtaining intestinal samples of both the graft and the recipient. Materials and Methods: We performed ITx in 10 pairs of piglets, each weighing 15-20kg. Procurement included an isolated segment of the small bowel, constituting a model of a living, related donor surgery. The recipient procedures included end-to-end anastomosis of vascular stumps, a proximal jejuno-jejunal anastomosis, and a distal modified Paul-Mikulicz ileostomy. The procedure differed from the classic Paul-Mikulicz ileostomy in that a common channel was created in a side-to-side fashion. Results: Vascular thrombosis occurred in three pigs, resulting in immediate loss of the graft. All other pigs underwent ITx successfully and survived for at least 1 wk (7-180 d). No pig experienced anastomotic leaks, ileus, or stoma-related complications. Moreover, this technique enabled us to obtain tissue samples of both the graft and the native ileum without disturbing the natural bowel conduit. Conclusions: The modified Paul-Mikulicz ileostomy was feasible in a swine model of ITx. It facilitated the collection of intestinal samples of both the graft and the recipient. ? 2010 Elsevier Inc. All rights reserved.en[SDGs]SDG3cefmetazole; methylprednisolone; tacrolimus; animal experiment; animal tissue; article; continuous infusion; controlled study; end to end anastomosis; graft failure; graft survival; ileostomy; ileum; intestine transplantation; male; nonhuman; priority journal; surgical technique; swine; thrombosis; Animals; Body Weight; Humans; Ileostomy; Immunosuppressive Agents; Intestinal Diseases; Intestine, Small; Living Donors; Male; Mesenteric Arteries; Mesenteric Veins; Parenteral Nutrition; Postoperative Period; Reperfusion; Swine; Tacrolimus; Transplantation, Homologousjournal article10.1016/j.jss.2009.05.040197757012-s2.0-78449273245