2004-05-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/684326摘要：過去30年亞蔬在發展適合高溫生長的蕃茄品系上有所貢獻。然而這些品系在著果率與產量上仍遠不及溫帶品系。我們的目標是在改善生長於夏日高溫蕃茄之產量並具有高量之茄紅素。要達成此目標需仰賴多種研究策略，本計劃將利用分子演化的方法來加以探索。目前有證據支持來自阿拉伯芥之熱休克蛋白HSP101是生存於高熱逆境下必須，用以獲得熱保護作用。此外粒線體內的熱休克蛋白MT-SHSP亦在高溫下被誘導出來並具有保護的功能。本計劃將選用不同之耐熱與不耐熱蕃茄品系，比較HSP101與MT-SHSP基因之天擇現象。使用不同軟體來分析DNA序列之多型性，核&#33527;酸歧異度並測試中性選擇假說。本研究可以釐清熱休克蛋白在耐熱與不耐熱蕃茄品系之間所居之角色。<br> Abstract: Developing tomato varieties can survive high temperature has been achieved by the effort of AVRDC in the past 30 years. However, the fruit setting and productivity is still far lag behind the temperate varieties. It is our goal to improve the production of tomato having high lycopene content under summer heat. To achieve these goal different strategies can be adopted and in this project molecular evolutionary method will be applied. Compelling evidence has been shown that HSP101 from Arabidopsis is essential for survival under severe heat stress and for the acquisition of thermotolerance. MT-sHSP, induced under heat, from tomato also has been shown having the molecular chaperone function. In this project we are going to compare the pattern of selection of HSP101 and MT-sHSP genes of both heat tolerant and heat intolerant varieties. Different software will be applied to analyze the polymorphism of DNA sequence, nucleotide diversity and test of neutral selection. Result from this study can clarify the role of heat shock protein plays among heat tolerant and heat intolerant varieties.蕃茄熱休克蛋白天擇DNA序列分析.tomatonatural selectionheat shock proteinDNA sequence analysis