2016-01-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/646659摘要：由於血腦障壁 (BBB) 這特殊結構的存在，使得藥物難以進入腦部，造成中樞神經 (CNS) 疾病治療的困難。超音波已被發現能有效改變 CNS 中血管的通透性，使得 BBB 暫時打開，提供藥物進入CNS 的治療窗口。本CARIBBBOU 計畫的主要目標就在於設計並製作一即時活體空穴作用(cavitation effect) 的控制器，用於使大分子的藥物或 DNA，能在超音波能量的協助下穿越 BBB 進入 CNS 中。本計畫預定要達到兩個主要成果：(1) 研究超音波打開 BBB 的物理與生物機轉，(2) 在活體中以超音波打開 BBB 增加治療用DNA 進入 CNS 的效率。<br> Abstract: Brain is a notoriously difficult organ for therapeutic agents to reach due to the presence of the blood brainbarrier (BBB), a specialized structure in the blood vessels and capillaries of central nervous system (CNS).The CNS-blood permeability can be significantly enhanced using ultrasound techniques, through thetemporal opening of the BBB, providing a promising strategy to increase delivery of therapeutic agent intothe brain. The aim of the CARIBBBOU project is to design and implement a real-time signal feedback forcavitation control to facilitate ultrasound-based gene therapy or drug delivery, with a particular focus on theBBB opening. Two specific devices will be designed for the following applications: (i) in-vitro: theidentification of the biophysical mechanisms underlying ultrasound and cavitation-facilitated uptake in cellsand BBB opening (using mimicking cell lines), and (ii) in-vivo: the enhancement of the BBB opening and thepenetration of therapeutic genes.