2011-01-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/659279摘要：收集各養殖區九孔病毒感染, 病毒分布情形和死亡率以及替代性魚種之發病和死亡率情形，可提供漁民各養殖區是否適合養殖之資料，作為預警之用。並應用於病材之快速診斷，縮短檢查所需時間並可在現場應用，因此可及早偵測水產生物是否感染病毒，避免引入帶病毒之魚種，減少病毒爆發及疫情擴散。實施方法為九孔&#30129;疹病毒之監測：主要進行不同品系之種貝所繁殖之仔代之不同生長期&#30129;疹病毒感染之檢查。採集病材以PCR技術進行九孔&#30129;疹病毒，將收集九孔檢體粹取DNA，以PCR技術進行九孔&#30129;疹病毒檢查。DNA經稀釋後以PCR技術進行定量試驗。並配合real time PCR作進一步確診。綜合上述病毒檢查和幼貝來源及成貝養殖等資料，可回朔檢討母貝來源，了解並建立優良品系資料庫，作為未來之種貝之用。九孔慢性及高死亡率病之病因探討：至各九孔養殖現場調查疫情及瞭解養殖狀況。採取九孔中貝慢性死亡病例，作細菌分離、病理學檢查、電子微鏡檢查及聚合酵素鏈鎖反應試驗及分析，以有效了解各養殖場感染之病因並研擬處理方式，以有效預防九孔疫病發生。替代性魚種疾病監測: 包括縐紋盤鮑和石斑至各養殖現場調查疫情及瞭解養殖狀況。採取死亡病例，作細菌分離、病理學檢查、電子微鏡檢查及聚合酵素鏈鎖反應試驗及分析，以有效了解各養殖場感染之病因並研擬處理方式，以有效預防替代性魚種疫病發生。<br> Abstract: Mass mortalities of abalone occurred among cultured abalone in northeastern Taiwan has been reported. Both adult and juvenile abalone suffered from the disease with high mortalities. Histological examination of moribund abalone indicated that the nerve system was the primary target tissue. Genome sequence of the virus collected from moribund animal was consistent with a herpes-type virus described from the oyster, Crassostrea virginica. In the study, a monitor system will be established to study in herpes-like virus infection of abalone in the farms. PCR technique will be applied to do this study. And the result of study will apply to the culture of abalone in the field. Further study about etiology of low rate mortality of abalone will be done. In this study, monitor of diseases associated with the alternated fish species including black abalone and grouper will be done to prevention the disease spread in the field and decrease the economic loss of farmers.九孔疾病監測abalonediseasesmonitor