2008-08-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/696672Breast cancer is the most common type of cancer for women and phenotypically complexities disease. Despite advances in early detection technique and the well-established treatment protocols for patients, cancer recurrence after therapy and long periods of remission is frequent. At that point, resistance to therapy is not only common but expected. Two hypothesis were suggested to explain the recurrence and drug resistance: the cancer dormancy and cancer stem cells. In recently, bone–marrow derived meschymal stem cells mesenchymal stem cell have been described to enhanced breast cancer cells metastatic ability. These data demonstrate that the tumor microenvironment facilitates metastatic spread by eliciting reversible changes in the phenotype of cancer cells. Although the phenomenon for cancer dormancy, or the hypothesis of cancer stem cells, or the microenvironment effect for metastasis were proposed to explain the tumor progression and recurrence, the general issue to treat breast cancer recurrence is to overcome the drug resistant. Therefore, how to set a useful system and criteria to screen and evaluate effective drugs in order to treat recurrent patient is the main topic in this proposal. In this proposal, we will try to make a personal primary cell culture for individual patient, and then, the procedure will carry out for drug screening. We believe that the experiments could give more information about the issue of drug resistant in the culture cells. The information will offer the basic message for the correlated patient who provided the biopsy, once the recurrence of tumor was occurred.