2017-08-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/713237Peri-implantitis is defined as an inflammatory process affecting the tissues around an osseointegrated implant in function. Although peri-implantitis is a common clinical phenomenon, no standard treatment protocol has yet been established because of limited knowledge on implant surface changes after bacterial contamination. Er: YAG laser irradiation has been reported to be effective for treating peri-implantitis. The objectives of this study are (1) to find out the optimal laser setting for in-vitro peri-implantitis; (2) to evaluate the morphological and elemental changes of titanium surfaces (sand-blasted and acid-etched; SLA) after various treatments; (3) to observe the adhesion and proliferation potential of preosteoblast cells on titanium surfaces after various treatments; (4) to quantify the residual endotoxin (lipopolysaccharide, LPS) on titanium surfaces after various treatments. Periodontal bacteria P. gingivalis biofilms are inoculated on commercially grade 4 pure titanium discs with SLA surface (15 mm in diameter, 2 mm in thickness, Ra=1.3) in vitro. They are divided into five groups, Group 1 receives no treatment and served as control. Group 2 receives P. gingivalis inoculation without treatment. Group 3 receives P. gingivalis inoculation, curette debridement and PBS irrigation. Group 4 receives P. gingivalis inoculation, curette debridement, PBS irrigation and ultrasonication. Group 5 receives P. gingivalis inoculation, curette debridement, PBS irrigation and Er: YAG laser treatment. The contact angle, surface roughness and hydrophilicity are studied. The adhesion and proliferation of human embryonic palatal mesenchymal (HEPM) cells are observed. The residual LPS is measured by Fourier transform infrared spectroscopy (FTIR), x-ray photoelectron spectroscopy (XPS) and limulus amebocyte lysate (LAL) assay. We study various factors associated with osseointegration on titanium surfaces, and these results may be beneficial for proposing an effective decontamination protocol in treating clinical peri-implantitis.Peri-implantitis； Er: YAG Laser； osseointegration； endotoxin