2021-08-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/654170腦部神經調節一直是近年全世界研究者研究的重點，其中又以體外機械力刺激來調 控腦功能之研究已有一些報導。由於機械力刺激腦部研究尚在萌芽階段，仍需更深 入探討與分析，因此，本實驗室計畫利用特殊的機械波裝備 -- 震波 (shockwave)，來發展獨特的開啟血腦屏障(blood-brain barrier or BBB) 技術，有 別於常見的利用超音波開啟血腦屏障技術，震波具有簡易設置、精準定位、低累積 能量、不易產熱與自由基等優勢。然而單獨使用震波仍有成功率不佳的問題。因此 本計畫首年目標為利用震波搭配超音波顯影劑 (ultrasound contrast agent) 來增強 空穴效應(cavitation)，以便達到百分之百的血腦屏障開啟率，並藉由調整震波強度 與顯影劑濃度等，來達到低傷害與高效能的血腦屏障開啟率。而後第二年將此技術 來發展新的開啟 blood-cerebrospinal fluid barrier (BCSFB)技術，有機會將藥物推 入腦內特殊的 cerebrospinal fluid (CSF) 循環。第三年則預定要將我們發展的開啟 BBB 與 BCSFB 技術，用於在腦中風之模式治療。希望將本研究團隊相關知識與 技術應用於相關腦部疾病之治療，以期提供一可能的腦刺激治療方案來造福病友 ，並提供基礎研究應用於疾病治療之範例。 Brain neuromodulation has been the focus of research by researchers all over the world in recent years. Among them, the use of mechanical stimulation to regulate brain function has been reported. Because mechanical stimulation on brain is still in its infancy, more research and analysis would be required. Our preliminary research has shown potential of employing shockwave to open blood- brain barrier (BBB), which is different from traditional ultrasound-mediated BBB opening. Shockwave has advantages of simple setting, precise positioning, low accumulated energy, and less thermal damage and free radicals. However, the success rate of using the shockwave to open the blood-brain barrier is still not ideal. Therefore, the goal of the first year of this project is to use shockwave with ultrasound contrast agents (UCAs) to enhance cavitation in order to achieve 100% blood-brain barrier opening rate. Low-injury and high-performance BBB opening rates are anticipated by adjusting shockwave intensity and UCA concentration. Next year, the technology will be used to develop a new technique for the blood cerebrospinal fluid barrier (BCSFB) opening, which enables us to delivery drugs into the CSF circulation of brain. The third year will use BBB and BCSFB opening techniques to promote drug delivery for further application in stroke model treatment. We hope to apply the knowledge and technology of our research team to the treatment of related brain diseases, in order to provide a possible brain stimulation treatment scheme to benefit patients, and provide good examples of translation medicine.震波血腦屏障空穴效應超音波顯影劑超音波腦脊液血腦脊液障壁shockwaveblood-brain barrierBBBblood-cerebrospinal fluid barrierBCSFBcerebrospinal fluidCSFultrasound contrast agentUCAultrasound