Yang T.-L.Lin F.-Y.Chen Y.-H.Chiu J.-J.Shiao M.-S.Tsai C.-S.Lin S.-J.YUH-LIEN CHEN2020-03-032020-03-0320110022-5142https://www.scopus.com/inward/record.uri?eid=2-s2.0-78649408796&doi=10.1002%2fjsfa.4163&partnerID=40&md5=cc3c3f1708a80689d16b182a9ea73c31https://scholars.lib.ntu.edu.tw/handle/123456789/468525BACKGROUND: Atherosclerosis and restenosis are inflammatory responses involving free radicals and lipid peroxidation and may be prevented/cured by antioxidant-mediated lipid peroxidation inhibition. Salvianolic acid (Sal B), a water-soluble antioxidant obtained from a Chinese medicinal herb, is believed to have multiple preventive and therapeutic effects against human vascular diseases. In this study the in vitro and in vivo inhibitory effects of Sal B on oxidative stress were determined.RESULTS: In human aortic endothelial cells (HAECs), Sal B reduced oxidative stress, inhibited low-density lipoprotein (LDL) oxidation and reduced oxidised LDL-induced cytotoxicity. Sal B inhibited Cu2+-induced LDL oxidation in vitro (with a potency 16.3 times that of probucol) and attenuated HAEC-mediated LDL oxidation as well as reactive oxygen species (ROS) production. In cholesterol-fed New Zealand White rabbits (with probucol as positive control), Sal B intake reduced Cu2+-induced LDL oxidation, lipid deposition in the thoracic aorta, intimal thickness of the aortic arch and thoracic aorta and neointimal formation in the abdominal aorta.CONCLUSION: The data obtained in this study suggest that Sal B protects HAECs from oxidative injury-mediated cell death via inhibition of ROS production. The antioxidant activity of Sal B may help explain its efficacy in the treatment of vascular diseases. ? 2010 Society of Chemical Industry.[SDGs]SDG3antioxidant; benzofuran derivative; copper; herbaceous agent; low density lipoprotein; reactive oxygen metabolite; salvianolic acid B; animal; aorta; article; chemistry; cholesterol intake; cytology; drug effect; endothelium cell; human; hypercholesterolemia; hyperplasia; intima; lipid metabolism; lipid peroxidation; metabolism; oxidative stress; pathology; phytotherapy; rabbit; Salvia miltiorrhiza; vascular disease; Animals; Antioxidants; Aorta; Benzofurans; Cholesterol, Dietary; Copper; Drugs, Chinese Herbal; Endothelial Cells; Humans; Hypercholesterolemia; Hyperplasia; Lipid Metabolism; Lipid Peroxidation; Lipoproteins, LDL; Oxidative Stress; Phytotherapy; Rabbits; Reactive Oxygen Species; Salvia miltiorrhiza; Tunica Intima; Vascular Diseases; Oryctolagus cuniculusjournal article10.1002/jsfa.4163208246802-s2.0-78649408796