2006-08-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/711603摘要：因應全球再生能源發展趨勢與微型電力系統需求與日遽增，本計畫擬結合無隔膜生物燃料電池技術與微製程發展「酵素型生物燃料電池陣列晶片」。此能源元件使用來源不虞匱乏的葡萄糖與氧氣作為燃料。考量人體植入應用的可能性，我們選定可於生理條件下操作的葡萄糖氧化酵素GOx與膽紅素氧化酵素BOD作為陽極與陰極的活性物質。燃料電池操作過程中，葡萄糖被生物陽極氧化所釋出的電子會經由外電路到達生物陰極參與氧分子的還原反應，而酵素、電極之間的電子傳遞則由媒介分子完成。然而為了避免兩極所用之媒介分子交互作用，具有軟側鍊的高分子將用來共價交聯媒介分子形成具有氧化還原能力之導電聚合物。將導電聚合物與酵素一併固定到電極表面便形成所謂的「導線架構酵素電極」，並可達成微型無隔膜燃料電池目標。本計畫擬利用光譜分析、表面結構鑑定與電化學量測探究導線架構酵素電極製備與生物燃料電池性能之間的關聯性，並同時進行電池操作條件的最適化。另一方面，本研究亦將應用微電極製程於玻璃或塑膠基版上進行串聯及並聯型式的電池陣列堆疊，以提高系統之輸出功率與增加被驅動元件之間的相容性。最後，我們將實際運用開發完成之燃料電池陣列晶片驅動低功率電子元件並進行<br> Abstract: By considering both global trend of renewable energy development and increasing need of micro power systems, this project plans to develop “enzymatic biofuel cell array on a chip” on the basis of membrane-free biofuel cell technology and microfabrication. The energy device proposed here will take advantage of glucose and oxygen as fuels, which are both abundant in nature. For the possibility of implanting the biofuel cell into human bodies, we will choose glucose oxidase (GOD) and bilirubin oxidase (BOD) that can be operated under the physiological condition as the active materials on the anode and the cathode, respectively. During fuel cell operation, the electrons released from the glucose oxidation at the bioanode will flow into the biocathode to trigger the reduction of oxygen through the external circuit. And the electron transfer between enzymes and electrodes will be accomplished by mediators. To prevent from interaction between the mediator systems of both electrodes, the polymers with a flexible side chain will be used to covalently crosslink mediators. Then redox polymers will be formed accordingly. Through immobilization of both redox polymer and enzyme on the surface of electrode, a “wired enzyme electrode” will be prepared, and the membrane-less fuel cell can be thus assembled. We will employ spectrum analysis, surface structural characterization, and electrochemical measurement to study the relationship between wired enzyme electrode’s preparation and biofuel cell’s performances. The operation condition of a biofuel cell will be optimized, too. Besides, this research will utilize microfabrication to stack cell array in series and/or in parallel on a glass or plastic substrate to form a biofuel cell array on a chip. The main purposes are to increase system’s power density and to enhance the compatibility with the devices that will be powered by the fuel cell chip. Finally, we will demonstrate the chips with fuel cell arrays by driving low-power electronic devices and will evaluate both performance and applicability of the chip-based fuel cells.生物燃料電池葡萄糖氧氣再生能源導線架構酵素電極biofuel cellglucoseoxygenrenewable energywired enzyme electrodes