2011-08-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/658336摘要：立克次體屬於α-Proteobacteria，為絕對細胞內維生，主要寄生或共生在真核宿主的細胞質內，於細胞質內行自由營生，寄主相當廣泛，包括脊椎動物、節肢動物、環節動物、原生動物和植物等。立克次體主要藉由經卵傳播或跨齡期傳播來傳遞後代，並可透過節肢動物吸血或叮咬而感染脊椎動物宿主。台灣近期已有自蜱和蚤分離出斑點熱病原體的研究報告，但尚無官方或學界發表的斑點熱立克次體病、人類單核細胞埃立克次體病和人類顆粒細胞無形體病等人類病例報告，相關生態和流行病學資料更是缺乏。本研究第一年將綜整通報疑似蟲媒病或鼠媒傳染病的流病資料，結合野鼠的血清抗體檢驗結果，利用地理資訊系統和空間統計描繪出台灣本島蟲媒立克次體病的地理熱區；同時發展非專一性立克次體抗體吸附排除技術供第二年檢驗使用。第二年於熱區採集外寄生蟲、野生動物血液及當地民眾血液，以細胞培養、分子和血清學方法分析立克次體感染種類和比例。第三年將改良建立不同類緣細胞株的細胞培養系統，以及分析系統間立克次體外膜蛋白的基因變異模式和細胞病理現象。本研究對於瞭解台灣立克次體疾病的現況和對於民眾可能造成的健康威脅相當有助益，且能同時建立台灣本土蟲媒立克次體之多樣性和立克次體病流行病學資料，加強立克次體基礎生物學研究。<br> Abstract: The order Rickettsiales belongs to the α-Proteobacteria and is comprised entirely ofobligate intracellular symbionts of eukaryotes. Hosts of rickettsia are very diverse, and at leastinclude vertebrates, arthropods, annelids, protozoans, and plants. Rickettsiae are sustained bytransovarial and transstadial transmission in arthropods, which can also infect vertebratesthrough blood-feeding. Recently, spotted fever group rickettsiae are isolated from ticks andfleas in Taiwan; however, human cases of rickettsioses, ehrlichioses, and anaplasmosis havenever been confirmed. Besides, related ecological and epidemiological studies are still verylimited. In the first year, we propose to create maps of hot spots of vector-borne rickettsialdiseases using geographic information system (GIS) and spatial analysis based on officialreportable diseases collated from Centers for Diseases Control, Taiwan and serum analyses ofwild rodents. Non-specific antiserum adsorption technique will also be developed. In thefollowing year, biological materials from ectoparasites, wild animals and local residentscollected in hot spots will be analyzed for rickettsial infection using a combination of cellculture and isolation systems, molecular techniques and serological methods. In the last year,we plan to improve the rickettsial isolation systems by compiling different cell lines, validatemutation and adaptation of rickettsial outer membrane proteins, and test cell pathogenesis.This project will contribute greatly to the understanding of current status of rickettsialdiseases, as well as their potential health risk to people in Taiwan. Besides, this study will alsosignificantly enhance our knowledge of biodiversity and epidemiology of vector-bornerickettsioses in Taiwan.熱區立克次體立克次體病斑點熱傳染病流行病學hot spotrickettsiarickettsiosesspotted feverinfectious diseaseepidemiology