KAI-WEN HUANGYang S.Y.Yu C.Y.Chieh J.J.Yang C.-C.Horng H.-E.Hong C.-Y.Yang H.-C.CHAU-CHUNG WU2020-06-192020-06-1920111550-7033https://www.scopus.com/inward/record.uri?eid=2-s2.0-80052886167&doi=10.1166%2fjbn.2011.1321&partnerID=40&md5=d72d6bf4353e2a5fc0f75f838f2eb946https://scholars.lib.ntu.edu.tw/handle/123456789/503654To achieve early-stage diagnosis, a high-sensitivity assay method is needed. As a biomarker, vascular endothelial growth factor (VEGF) has played a growing role in diagnosing and treating hepatocellular carcinoma (HCC). In this work, an immunomagnetic reduction (IMR) through bio-functionalized magnetic nanoparticles and a high-temperature superconducting-quantum-interference-device magnetometer were utilized for quantitative detection of low-concentration VEGF in serum from rats with HCC. The precision and accuracy of IMR on VEGF were characterized. Further, the results of assaying VEGF in the serum of rats were compared with those of using enzyme-linked immunosorbant assay (ELISA). It was found the correlations between the detected VEGF concentration in the rat serum and tumor burdens were 0.99 and 0.90 for IMR and ELISA, respectively, within the range from 2 pg/ml to 8000 pg/ml of VEGF concentration. Copyright ? 2011 American Scientific Publishers All rights reserved.[SDGs]SDG3Animal model; Assay methods; Early-stage diagnosis; Hepatocellular carcinoma; High temperature; High-sensitivity; Immunosorbant assay; Low concentrations; Magnetic nanoparticles; Quantitative detection; Rat serum; Vascular endothelial growth factor; Animals; Body fluids; High temperature superconductors; Nanoparticles; Rats; Tumors; Concentration (process); magnetic nanoparticle; vasculotropin; magnetite nanoparticle; tumor marker; vascular endothelial growth factor A, rat; vasculotropin A; animal experiment; animal model; animal tissue; article; cancer diagnosis; enzyme linked immunosorbent assay; immunoassay; immunomagnetic reduction; intermethod comparison; limit of detection; liver cancer; magnetism; male; nonhuman; protein blood level; rat; temperature; tumor volume; animal; blood; blood analysis; comparative study; evaluation; liver tumor; methodology; nanotechnology; pathology; tumor volume; Wistar rat; Animals; Blood Chemical Analysis; Enzyme-Linked Immunosorbent Assay; Liver Neoplasms, Experimental; Magnetics; Magnetite Nanoparticles; Male; Nanotechnology; Rats; Rats, Wistar; Tumor Burden; Tumor Markers, Biological; Vascular Endothelial Growth Factor Ajournal article10.1166/jbn.2011.1321218704572-s2.0-80052886167