Wang, Jin-TownJin-TownWangChang C.-S.Lee, Cha-ZeCha-ZeLeeJYH-CHIN YANGLin J.-T.Wang T.-H.2019-09-022019-09-0219980006-291Xhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0032539757&doi=10.1006%2fbbrc.1998.8271&partnerID=40&md5=4fa0a838557652570ed5f7791cd687f2https://scholars.lib.ntu.edu.tw/handle/123456789/417219This study attempted to identify a possible antibody response to Helicobacter pylori, which is associated with patients with adeno-carcinoma of the stomach. By using proteins of H. pylori as the antigen, pooled sera from gastric cancer and non-cancer patients were used as the first antibody for Western blot analysis. Antibody responses to a 26 kD secreted protein were observed in pooled cancer sera, but not in pooled sera from non-cancer patients. The protein was purified, while amino acid sequences revealed that it was a H. pylori species specific protein. The gene of this protein was cloned and a recombinant protein was expressed in E. coli. In addition, an antibody to the recombinant protein was tested in each individual patient using Western blot analysis. None of the forty non-gastric cancer patients were positive for the antibody to the recombinantly expressed 26 kD species specific protein. Meanwhile, six of the twenty four cancer patients tested positive (0/40 vs 6/24, p < 0.01). Results presented herein demonstrate that the species specific protein of H. pylori can be useful in detecting H. pylori associated with adenocarcinoma of the stomach.en-US[SDGs]SDG3bacterium antibody; recombinant protein; adult; aged; amino acid sequence; antibody response; article; clinical article; controlled study; Escherichia coli; female; Helicobacter pylori; human; immunoblotting; male; molecular cloning; priority journal; protein purification; stomach adenocarcinoma; Taiwan; Bacteria (microorganisms); Escherichia coli; Helicobacter pylorijournal article10.1006/bbrc.1998.827195149292-s2.0-0032539757