Tandon, KshitijKshitijTandonWan, Min-TaoMin-TaoWanYang, Chia-ChinChia-ChinYangSHAN-HUA YANGBaatar, BayanmunkhBayanmunkhBaatarChiu, Chih-YuChih-YuChiuTsai, Jeng-WeiJeng-WeiTsaiLiu, Wen-ChengWen-ChengLiuNg, Chen SiangChen SiangNgTang, Sen-LinSen-LinTang2021-07-262021-07-26202100489697https://www.scopus.com/inward/record.uri?eid=2-s2.0-85105351803&doi=10.1016%2fj.scitotenv.2021.147433&partnerID=40&md5=94231c4c3defe077c2e6379cbe17d020https://scholars.lib.ntu.edu.tw/handle/123456789/573354Microbial communities are considered to be functionally redundant, but few studies have tested this hypothesis empirically. In this study, we performed an in situ reciprocal transplant experiment on the surface and bottom waters of two lakes (Tsuei-Feng (T) and Yuan-Yang (Y)) with disparate trophic states and tracked changes in their microbial community composition and functions for 6 weeks using high-throughput sequencing and functional approaches. T lake's surface (Ts) and bottom (Tb) water active bacterial community (16S rRNA gene-transcript) was dominated by Actinobacteria, Bacteroidia, and Cyanobacteria, whereas Y lake's surface (Ys) and bottom (Yb) water had Gammaproteobacteria, Alphaproteobacteria, and Bacteroidia as the dominant classes. The community composition was resistant to changes in environmental conditions following the reciprocal transplant, but their functions tended to become similar to the incubating lakes' functional profiles. A significant linear positive relationship was observed between the microbial community and functional attributes (surface: R2 = 0.5065, p < 0.0001; bottom: R2 = 0.4592, p < 0.0001), though with varying scales of similarity (1-Bray Curtis distance), suggesting partial functional redundancy. Also, the entropy-based L-divergence measure identified high divergence in community composition (surface: 1.21 ± 0.54; bottom: 1.17 ± 0.51), and relatively low divergence in functional attributes (surface: 0.04 ± 0.01; bottom: 0.04 ± 0.01) in the two lakes' surface and bottom waters, providing further support for the presence of partial functional redundancy. This study enriches our understanding of community functional relationships and establishes the presence of partial functional redundancy in freshwater ecosystems. ? 2021 Elsevier B.V.Aquatic organisms; Bacteria; Redundancy; RNA; Aquatic microbiome; Bacterial community composition; Bacterial functional profile; Bottom water; Community composition; Community IS; Community-function relationship; Functional redundancy; Microbial communities; Partial functional redundancy; Lakes; bacterium; community composition; environmental conditions; lake ecosystem; lake water; microbial community; Actinobacteria; Alphaproteobacteria; aquatic species; Article; Bacteroidia; controlled study; cyanobacterium; freshwater species; Gammaproteobacteria; lake; microbial community; microbial diversity; microbiome; nonhuman; priority journal; species composition; genetics; microbiology; microflora; phylogeny; Actinobacteria; Alphaproteobacteria; Bacteria (microorganisms); Cyanobacteria; Gammaproteobacteria; RNA 16S; Lakes; Microbiota; Phylogeny; RNA, Ribosomal, 16S; Water Microbiology[SDGs]SDG15Aquatic organisms; Bacteria; Redundancy; RNA; Aquatic microbiome; Bacterial community composition; Bacterial functional profile; Bottom water; Community composition; Community IS; Community-function relationship; Functional redundancy; Microbial communities; Partial functional redundancy; Lakes; bacterium; community composition; environmental conditions; lake ecosystem; lake water; microbial community; Actinobacteria; Alphaproteobacteria; aquatic species; Article; Bacteroidia; controlled study; cyanobacterium; freshwater species; Gammaproteobacteria; lake; microbial community; microbial diversity; microbiome; nonhuman; priority journal; species composition; genetics; microbiology; microflora; phylogeny; Actinobacteria; Alphaproteobacteria; Bacteria (microorganisms); Cyanobacteria; Gammaproteobacteria; RNA 16S; Lakes; Microbiota; Phylogeny; RNA, Ribosomal, 16S; Water Microbiologyjournal article10.1016/j.scitotenv.2021.147433339715972-s2.0-85105351803