口腔生物研究所CHANG, BEI-ENBEI-ENCHANG�i�ʮ�2008-12-292018-07-092008-12-292018-07-091999http://ntur.lib.ntu.edu.tw//handle/246246/94752Cold-shock domain proteins in vertebrates contain a highly conserved domain which is related to the Escherichia coli cold-shock proteins. Here we report the cloning of a cold- shock domain protein from zebrafish embryo. Using the combination of PCR techniques with degenerate primers, 5 P RACE and 3 P RACE, the full length cDNA of a cold-shock domain protein in the zebrafish embryo was successfully cloned without constructing and screening a library. Determined from the deduced amino acid sequence, this protein is most similar to Xenopus, FRGY 1, and this newly cloned zebrafish gene was therefore designated as zfY1. szlig; 1999 Elsevier Science B.V. All rights reserved.en-USCold-shock domainY boxZebrafishRapid amplification of cDNA ends