2020-08-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/706575"目前學界對於陰道滴蟲對人類陰道上皮細胞之附著性有相當大的爭議。我們檢視了 陰道滴蟲附著性相關研究，發現其中數個關鍵性文獻，在實驗設計或結果解讀上有 相當落差。在前期實驗裏，我們著手建立新的研究模式，以動態觀點佐以電子顯微 鏡觀察，細胞附著力及生化特性分析， 來探討陰道滴蟲之行為，生化特性與附著 力之關連。本計劃目標在於利用所建立的研究模式，釐清陰道滴蟲附著於人類陰道 上皮細胞之調控機制，及依此探討寄生蟲與宿主互動的生化免疫反應。此一研究有 助於瞭解參與在陰道滴蟲與宿主互動的蛋白質分子生化及生理特性，有助於疾病之 治療或疫苗之研發。" "Current observations addressing cytoadherence of T. vaginalis onto human epithelial cells derived from urogenital tracts have often been controversial and conclusions arguable. In the preliminary study, an assay system exploring conventional binding assays in conjunction with real-time and scanning microcopies as well as biochemical characterizations was developed to study the dynamics of host-parasite interactions like never before. We found that motility rather than the reputed adhesins or galactosides are crucial for the outcome of the parasite infection, and acetylation of on the surface of recurrent flagellum reversely correlates with the motility of the parasite. In this proposal, an integrated experimental scheme will be presented for elucidating cellular mechanism that dominates the parasite-host interactions. Hopefully, our efforts shall lead the way onto future development of new drugs or identification of vaccine candidates."陰道滴蟲蛋白質乙醯化組蛋白去乙醯化&#37238蛋白質磷酸化附著力人類陰道 上皮細胞Trichomonas vaginaliscytoadherenceacetylation, mobilityrecurrent flagellumgalectins.