Correlation of cytogenetic results with immunophenotype, genotype, clinical features, and ras mutation in acute myeloid leukemia A study of 235 Chinese patients in Taiwan

HWEI-FANG TIENWang C.-H.Lin M.-T.Lee F.-Y.Liu M.-C.Chuang S.-M.YAO-CHANG CHENMING-CHING SHENLin K.-H.Lin D.-T.2021-01-042021-01-0419950165-4608https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028810735&doi=10.1016%2f0165-4608%2895%2900084-4&partnerID=40&md5=f2f666a556c0b6b5a2350b53ec9d4ba2https://scholars.lib.ntu.edu.tw/handle/123456789/537564Of 235 consecutive patients with de novo acute myeloid leukemia (AML), clonal chromosomal abnormalities were detected in 151 (64%) of them. Twenty-four of the 71 patients with M2 AML had t(8;21), 35 of the 36 M3 patients had t(15;17), and 11 of the 45 M4 leukemia disclosed inv(16). Six of the eight patients with 11q23 abnormality had M4 or M5 subtype of leukemia. The incidence of t(15;17) and t(8;21) was higher in our patients than in patients from most Western countries. Immunophenotyping was performed on 197 patients. Patients with t(15;17) were associated with negativity to HLA-DR, CD11b, and CD34. Patients with t(8;21) expressed CD13 and CD33 less frequently than other patients, but all showed CD15 positivity. Coexpression of lymphoid-associated antigens on the leukemic blasts was detected in 52 patients (26%), including all 7 patients with t(9;22), 3 of the 8 patients with t/del(11)(q23), 2 of the 25 patients with t(15;17), and 2 of the 22 patients with t(8;21). Seven (35%) of the 20 patients coexpressing lymphoid markers showed immunoglobulin heavy chain or T-cell receptor β-chain gene rearrangements, while only 2 (4%) of the 53 patients without lymphoid antigen expression did so. Patients with inv(16), t(8;21), and t(15;17) had a better prognosis than other patients. Of all surface antigens tested, only CD15, CD11b, and HLA-DR were of prognostic value: CD15 with a higher complete remission (CR) rate and CD11b or HLA-DR with a shorter CR duration. N-ras mutations were detected in 7 (18%) of the 40 patients in the study, including two of the three patients with inv(16). This study demonstrated differences in clinical features, immunophenotypes, and genotypes among different cytogenetic subgroups. ? 1995.[SDGs]SDG3cd11b antigen; cd15 antigen; cd33 antigen; cd34 antigen; HLA DR antigen; immunoglobulin heavy chain; leukocyte antigen; lymphocyte antigen; microsomal aminopeptidase; t lymphocyte receptor beta chain; acute granulocytic leukemia; adolescent; adult; aged; article; blast cell; cancer regression; child; chinese; chromosome 11q; chromosome analysis; chromosome inversion; chromosome rearrangement; chromosome translocation; chromosome translocation 15; chromosome translocation 8; chromosome translocation 9; female; gene mutation; genotype; human; human cell; immunophenotyping; infant; major clinical study; male; oncogene ras; priority journal; Adolescent; Adult; Aged; Aged, 80 and over; Child; Child, Preschool; Chromosome Aberrations; Female; Genes, ras; Genotype; Human; Immunophenotyping; Infant; Leukemia, Myelocytic, Acute; Male; Middle Age; Mutation; Prognosis; Support, Non-U.S. Gov'tCorrelation of cytogenetic results with immunophenotype, genotype, clinical features, and ras mutation in acute myeloid leukemia A study of 235 Chinese patients in Taiwanjournal article10.1016/0165-4608(95)00084-474974452-s2.0-0028810735