Tang W.Li S.Liu Y.Wu J.-C.Pan M.-H.Huang M.-T.MIN-HSIUNG PANPan, M.-H.M.-H.Pan2019-07-122019-07-12201716134125https://scholars.lib.ntu.edu.tw/handle/123456789/413624Scope: Functional foods can be used alone or in combination with existing therapies in preventing and treating type 2 diabetes (T2D). Trans-2,3,5,4¡¬-tetrahydroxystilbene 2-O-£]-glucopyranoside (trans-THSG), a dominant bioactive compound from Polygonum multiflorum (PM)¡Xa popular medicinal food in Asia, has attracted increasing research interests due to its strong antioxidant activity. The content of naturally occurring cis-THSG (cis-2,3,5,4¡¬-tetrahydroxystilbene 2-O-£]-glucopyranoside) was very low in PM root, but was prepared in this study by mimicking the traditional process of PM. The anti-diabetic effects of trans- and cis-THSG were evaluated in T2D to search for more efficacious food ingredient(s). Methods and results: Trans-THSG was chromatographically purified from PM roots and cis-THSG was prepared with our innovative process via exposure of trans-THSG to UV-light. The anti-diabetic effects of both THSGs were tested with HFD-induced male CF-1 diabetic mice. Cis-THSG was found more effective than trans-THSG in hypoglycemic effect and in ameliorating glucose intolerance and insulin resistance. In HepG2 cells, cis-THSG also demonstrated more potent activity than trans-THSG in suppressing transcription of phosphoenopyruvate carboxykinase (PEPCK). Conclusion: Cis-THSG can be an enriched bioactive ingredient in PM roots from post-processing and is significantly more effective against hyperglycemia than trans-THSG. One of the effective pathways was through inhibition of PEPCK. ? 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim2,3,5,4¡¬-tetrahydroxystilbene glycosideHypoglycemic activityPEPCKPolygonum multiflorumType 2 diabetes[SDGs]SDG32,3,5,4'-tetrahydroxystilbene 2-O-beta-D-glucopyranoside; antidiabetic agent; enzyme inhibitor; glucoside; PCK2 protein, human; phosphoenolpyruvate carboxykinase (ATP); stilbene derivative; animal; antagonists and inhibitors; chemistry; experimental diabetes mellitus; Fallopia multiflora; genetics; Hep-G2 cell line; human; insulin resistance; lipid diet; male; non insulin dependent diabetes mellitus; Animals; Diabetes Mellitus, Experimental; Diabetes Mellitus, Type 2; Diet, High-Fat; Enzyme Inhibitors; Fallopia multiflora; Glucosides; Hep G2 Cells; Humans; Hypoglycemic Agents; Insulin Resistance; Male; Phosphoenolpyruvate Carboxykinase (ATP); Stilbenesjournal article10.1002/mnfr.2016008712-s2.0-85013851088https://www.scopus.com/inward/record.uri?eid=2-s2.0-85013851088&doi=10.1002%2fmnfr.201600871&partnerID=40&md5=48abd828fd0227a63eb5540249c27910